Sequencing of 16S−23S rRNA internal transcribed spacer and its application in the identification of Nocardia seriolae by polymerase chain reaction |
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| Authors: | Tomoya Kono Tsuyoshi Ooyama Shih-Chu Chen & Masahiro Sakai |
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| Institution: | United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, Japan;Miyazaki Prefectural Fisheries Experimental Station, Miyazaki, Japan;Veterinary Department, National Pingtung University of Science and Technology, Pingtung, Taiwan;Faculty of Agriculture, Miyazaki University, Miyazaki, Japan |
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| Abstract: | A method for the diagnosis of nocardiosis in yellowtail (Seriola quinqueradiata), using polymerase chain reaction (PCR), was developed in this study. Primers specific for Nocardia seriolae were synthesized based on the alignment of 16S?23S rRNA internal transcribed spacer region sequences of N. seriolae. The primers did not amplify specific PCR product from other fish pathogens. However, two and three fishes could be diagnosed as infected with N. seriolae by clinical signs and bacterial isolation. PCR amplification of N. seriolae by specific primers detected six infected fishes. Thus, the primers used in this study are useful in detecting nocardiosis in fish. |
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| Keywords: | nocardiosis 16S–23S ITS PCR diagnosis |
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