水稻条纹叶枯病的研究 Ⅳ.病叶细胞的病理变化

在水稻条纹叶枯病叶超薄切片的电镜检查中,可见一些叶肉细胞和维管束鞘细胞中叶绿体有不同程度的降解和淀粉粒累积,一些细胞不同程度坏死,叶肉细胞、维管束鞘细胞以及伴胞的细胞质或液泡中有一个或多个蛋白体存在,一些伴胞中有特异性的非外壳蛋白存在,一些细胞中有一些不定形的粒状内含体或砂状结构,而在病株的不同叶位,这种砂状结构数量不同,以+1叶所见最多.研究结果还佐证了前文有关叶绿体中淀粉粒的过量累积可能引起叶绿体的破坏及其后叶片褪绿病斑形成的假说,并推测这可能是同化产物的运输通道受到影响的结果.

Studies on Rice Stripe in China IV. Pathological Changes of Rice Leaf Cell

Results from the electron microscopic observation of thin—sectioned leaf tissues showed that chloroplasts in some mesophyll and bundle sheath cells of rice {Oryza sativa) leaves infected by rice stripe virus degenerated and were filled with starch grains variously. Cell necrosis was observed and one or several protein bodies could be found in the cytoplasm or vocules of mesophyll, bundle sheath and companion cells. Specific non—capsid protein could be found in the diseased companion cells. Amorphous granular inclusion bodies or sandy structures were observed in various cells such as mesophyll, bundle sheath and companion cells, particularly commonly in those of the newly matured leaf (+1 leaf) of rice plant. The hypothesis that the degeneration of chloroplasts and subsequent formation of leaf chlorotic spots were closely related to an overaccumulation of starch grains in them was further confirmed and which might result from an obstruction of photosynthate transportation system by virus infection.