Characterization of a monoclonal antibody against desmoplakin 1 for use in dogs |
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Authors: | Nakamura Yuka Nakashima Ko Kano Rui Watanabe Shinichi Hasegawa Atsuhiko |
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Affiliation: | Department of Veterinary Internal Medicine;, and Department of Pathobiology, Nihon University, School of Veterinary Medicine, Kanagawa, Japan; Department of Dermatology, Teikyo University, School of Medicine, Tokyo, Japan |
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Abstract: | Abstract Skin biopsy specimens from face, axilla, abdomen and thigh, mucocutaneous tissues from anus and vagina, and oral mucosa from six healthy Beagle dogs were examined for desmoplakin (Dsp) immunoreactivity using immunoblotting and immunohistochemical analysis. With immunoblotting using mouse antihuman Dsp 1 monoclonal antibody (DP2.17), a band was detected at 250 kDa in all the extracts as in normal human skin samples, although no band was detected at 210 kDa, suggesting that monoclonal antibody DP2.17 recognizes canine Dsp 1 but not Dsp 2. Moreover, the desmosome regions of all specimens were stained with DP2.17 using immunohistochemical analysis. From these results, DP2.17, developed for the examination of human skin, might be suitable for the investigation of Dsp-related skin disorders in dogs. |
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Keywords: | desmoplakin DP2.17 dog epithelial tissue immunoblot analysis immunohistochemical analysis |
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