假单胞菌对香蕉枯萎病菌的抑制作用

从已感染香蕉枯萎病的果园中分离到1株对香蕉枯萎病菌（Fusarium oxysporum f.sp.cubense）抑制作用很好的菌种，命名为G1。采用固体和液体培养法从不同角度证实了G1对香蕉枯萎病菌生长的抑制作用。结果表明，G1的发酵液、无菌滤液、挥发性物质、非挥发性代谢产物的平均抑菌率分别为92.5%、27.4%、73.8%、57.7%，可见抑制作用与活菌体有直接关系，其中产生挥发性物质尤其重要。液体培养的病原菌浓度为1.0×107cfu/mL经G1作用10d后，取样镜检发现G1通过抑制病原菌菌丝正常生长以至不能产孢，从而导致菌丝消融；通过吸附在孢子的周围，融解细胞壁，造成原生质泄露致使孢子死亡。

Inhibition of Pseudomonas sp. against Fusarium oxysporum f. sp. cubense in banana

An effective antagonistic bacterium against Fusarium oxysporum f.sp. cubense was isolated from the banana garden that was infected with wilt disease. The bacterium was named as G1. The experiments confirmed that G1 could inhibit the growth of the pathogen at different points by solid and liquid cultures. The results showed that the average inhibitory rates of the culture solution, sterile solution, volatile substance and non volatile matter were 92.5%, 27.4%, 73.8% and 57.7%, respectively. Inhibition was closely related to viable cells, and the volatile substances were particularly important. After 10 days of reaction in tubes, G1 inhibited the growth of the pathogen and its conidial germination, broken down hyphae. By attaching to the spores of the pathogen, it broke down the cell walls and disclose the protoplasm and finally lead the spores to die.