啤酒花根癌土壤杆菌生物型的鉴定及生物防治的初步探讨

啤酒花根癌病在我国发生普遍,是影响啤酒花生产的重要病害。从啤酒花主要生产基地新疆、山东、浙江等地分离到61个菌株。通过培养性状、染色反应,以及14～15个菌株的生理生化性状和12个菌株致病性的测定,确定啤酒花根癌病病原菌为根癌土壤杆菌Agrobacteria tumefaciens(E.F.Smith &Jownsend)Conn属生物型1、病原菌菌株对K84(Agrobacterium radiobacter)菌株的细菌素敏感,各菌株间敏感性的强弱不一。盆裁番茄和啤酒花初步生防试验表明,用K84与敏感性强的菌株按1:1比例混合接种,番茄和啤酒花都不产生瘿瘤;而敏感性弱的菌株需用3:1才能防止瘿瘤的产生。

IDENTIFICATION OF BIOTYPE OF AGROBACTERIUM TUMEFACIENS OF HOP AND PRELIMINARY STUDIES ON BIOLOGICAL CONTROL

Sixty one isolates were isolated from gall samples of hop and from soil of infected fields in Xinjiang, Shandong and Zhejiang provinces. According to the cultural characters and stained reaction of 14-15 isolates, physiological and biochemical characters and pathogenicity of 12 isolates, these isolates were identified as Agrobacterium tumefaciens and belonged to Biotype I.The pathogenic Agrobacteria isolates were sensitive to Agrocin 84 producing by A. radiobacter K-84, but varied in some extent of sensitivity. Biological control tests with K-84 in. greenhouse on hod and tomato plant, indecated no gall production or the size and number of gall decreased with different proportions of K-84.The isolates of strong sensitivity in vitro gave no gall production on hop and tomato at 1:1 (antagonist:pathogen) ratio while the isolates of weak sensitivity in vitro showed no gall production at 3:1 ratio.