Autofluorescence as a diagnostic tool for the infection of alders by Frankia |
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Authors: | Neil R. McEwan |
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Affiliation: | (1) Department of Botany, University of Glasgow, Glasgow, G12 8QQ, Scotland;(2) Crop Sciences Section, Department of Agriculture for Northern Sweden, Swedish University of Agricultural Sciences, S-903 04 Umeå, Sweden;(3) Land Resources Department, Scottish Agricultural College, Craibstone Estate, Aberdeen, AB21 9SJ, Scotland |
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Abstract: | The early stages in the infection process between Frankia and the roots of its host plants are poorly understood. At present, there is relatively little known about the infection process in plants after the initial stage of root hair curling, but before a nodule may be visualised by eye. This paper presents a novel method of screening plant-microbe interactions, which is non-lethal to the plant, on the basis of a change in the autofluorescent pattern in the plants root system when viewed using fluorescent microscopy with an excitation wavelength of 490 nm. |
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