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Effects of dietary humic acid on growth performance,haemato‐immunological and physiological responses and resistance of Rainbow trout,Oncorhynchus mykiss to Yersinia ruckeri
Authors:Sevdan Yılmaz  Sebahattin Ergun  Ekrem Şanver Çelik  Murat Yigit
Affiliation:1. Department of Aquaculture, Faculty of Marine Sciences and Technology, ?anakkale Onsekiz Mart University, ?anakkale, Turkey;2. Department of Basic Science, Faculty of Marine Sciences and Technology, ?anakkale Onsekiz Mart University, ?anakkale, Turkey
Abstract:This study investigated the effects of dietary humic acid sodium salt on growth performance, haemato‐immunological and physiological responses, and resistance of rainbow trout, Oncorhynchus mykiss to Yersinia ruckeri. The experimental fish were divided into four groups; three of them were fed with humic acid incorporated diets (0.3% H3, 0.6% H6, 1.2% H12) and an additive free basal diet served as the control. Growth performance and haematological parameters of rainbow trout were not affected by humic acid supplemented diets (p > 0.05). However, dietary humic acid especially with 0.6% incorporation significantly increased stomach pepsin, intestinal trypsin and lipase activities p < 0.05. Following 60 days of feeding trial, fish were challenged with Yersinia ruckeri for 20 days. At the end of the challenge period, significantly higher (p < 0.05) survival rates were found in the 6% humic acid group compared to all other experimental treatment. Thus humic acid might replace antibiotics in diets for rainbow trout to control yersiniosis.
Keywords:digestive enzyme  humic acid  immunological responses  rainbow trout  yersiniosis
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