| 复合酶法提取山茱萸多糖的工艺条件优化 |
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| 引用本文： | 程俊文,贺亮,吴学谦,付立忠,胡传久,吴庆其,李海波,魏海龙.复合酶法提取山茱萸多糖的工艺条件优化[J].中国林副特产,2010(3):1-4. |
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| 作者姓名： | 程俊文 贺亮 吴学谦 付立忠 胡传久 吴庆其 李海波 魏海龙 |
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| 作者单位： | 浙江省林业科学研究院,浙江省森林资源生物与化学利用重点实验室,浙江,杭州,310023;丽水市食用菌研究开发中心,浙江益圣菌物发展有限公司,浙江,丽水,323000 |
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| 基金项目： | 浙江省创新团队建设与人才培养项目，浙江省林业科技项目 |
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| 摘 要： | 在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化复合酶法提取山茱萸多糖的工艺条件。响应面分析结果表明,由纤维素酶2.0%、果胶酶2.0%、中性蛋白酶1.5%组成的复合酶,在浸提时间69min,浸提温度50℃,pH值3.8时,酶法提取山茱萸多糖的得率最高,山茱萸多糖实际最大提取得率为5.54%。
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| 关 键 词： | 山茱萸多糖 复合酶 响应面法 提取 |
Optimization of Extracting Technique of Polysaccharide from Fructus Corni with Compound Enzymes |
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| Cheng Junwen,He Liang,Wu Xueqian,Fu Lizhong,Hu Chuanjiu,Wu Qingqi,Li Haibo,Wei Hailong.Optimization of Extracting Technique of Polysaccharide from Fructus Corni with Compound Enzymes[J].Forest By-product and Speciality in China,2010(3):1-4. |
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| Authors: | Cheng Junwen He Liang Wu Xueqian Fu Lizhong Hu Chuanjiu Wu Qingqi Li Haibo Wei Hailong |
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| Institution: | Cheng Junwen1,2,He Liang1,Wu Xueqian1,Fu Lizhong1,Hu Chuanjiu1,Wu Qingqi1,Li Haibo1,Wei Hailong1,2(1.Zhejiang Forestry Academy,Key Laboratory of Biological and Chemical Utilization of Forest Resources,Hangzhou,Zhejiang,310023,2.Lishui Edible Fungi R & D Center,Zhejiang Essence Fungi Development Co.,Ltd,Lishui,323000) |
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| Abstract: | Based on the results of one-factor-at-a-time-technique method, a threeqevel Box-Behnken factorial design was employed combining with response surface methodology （RSM） to optimize the extraction conditions for the yield of Fructus Corni polysaccharide with compound enzymes. The optimal extract conditions are as follows. 2.0 % cellulase, 2.0 % pectic enzyme and 1.5% neutral protease, enzymolysis temperature 50℃, treatment 69 min at pH3.8. The extraction rate of Fructus Corni polysaccharide is 5.54% under these conditions. |
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| Keywords: | Fructus Corni Polysaccharide Compound enzymes Response surface analysis methodology Extraction |
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