| Molecular Cloning and Sequence Analysis of Chitin Synthase cDNA from Mamestra brassicae （L.） （Lepidoptera： Noctuidae） Cuticle |
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| 作者姓名： | CHANG Xiaojiao FAN Dong PIAO Donghua |
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| 作者单位： | College of Agriculture,Northeast Agricultural University,Harbin 150030,China |
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| 基金项目： | Natural Science Foundation of Heilongjiang Province，Scientific and Technical Innovation Fund of Harbin，Northeast Agricultural University Research Fund |
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| 摘 要： | Chitin is the most widespread amino polysaccharide in nature. Chitin synthase （CHS） plays an important role in chitin formation in the cuticle and the peritrophic membrane （PM） lining the midgut. Total RNA was isolated from the cuticle of Mamestra brassicae （L.） fourth instar larva, cDNA sequence was cloned by RT-PCR and Rapid Amplification of cDNA Ends （RACE）. cDNA 5 220 bp in length, contained an open reading frame of 4 704 bp coding for a polypeptide of 1 567 amino acid residues with a predicted molecular weight of 178.3 ku and its pI was 6.42. The deduced amino acid sequence from Mi brassicae （L.） shared the high level of identity with chitin synthase sequences from other insects, especially lepidopteran insects, cDNA sequence has been deposited with GenBank under accession No. GQ281761
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| 关 键 词： | Mamestra brassicae （L.） cuticle chitin synthase cloning sequence analysis |
Molecular Cloning and Sequence Analysis of Chitin Synthase cDNA from Mamestra brassicae (L.) (Lepidoptera: Noctuidae) Cuticle |
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| CHANG Xiaojiao,FAN Dong,PIAO Donghua.Molecular Cloning and Sequence Analysis of Chitin Synthase cDNA from Mamestra brassicae (L.) (Lepidoptera: Noctuidae) Cuticle[J].Journal of Northeast Agricultural University,2010,17(3):12-18. |
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| Authors: | CHANG Xiaojiao FAN Dong PIAO Donghua |
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| Institution: | College of Agriculture,Northeast Agricultural University,Harbin 150030,China |
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| Abstract: | Chitin is the most widespread amino polysaccharide in nature.Chitin synthase (CHS) plays an important role in chitin formation in the cuticle and the peritrophic membrane (PM) lining the midgut.Total RNA was isolated from the cuticle of Mamestra brassicae (L.) fourth instar larva.cDNA sequence was cloned by RT-PCR and Rapid Amplification of cDNA Ends (RACE).cDNA,5 220 bp in length,contained an open reading frame of 4 704 bp coding for a polypeptide of 1 567 amino acid residues with a predicted molecular wei... |
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| Keywords: | Mamestra brassicae (L ) cuticle chitin synthase cloning sequence analysis |
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