Absorption,Distribution and Pathological Injury in Mice Due to Ricin Poisoning Via the Alimentary Pathway |
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| Authors: | Na Dong Zheng Li Qian Li Junhua Wu Peiyuan Jia Yuxia Wang Zhongcai Gao Gang Han Yifan Wu Jianping Zhou Junjie Shan Hua Li Wenqing Wei |
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| Institution: | 1. General Hospital of Beijing Military Command, Beijing 100700, China;2. Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China;3. School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China |
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| Abstract: | The aim of this work was to investigate the potential interactions between intestinal absorbance and ricin poisoning. The Caco-2 cell monolayer and everted intestinal sac (VEIS) models were used. The distribution of ricin in CD-1 mice intoxicated with 0.1 mg/kg of ricin intragastrically was determined by immunohistochemistry. The results showed that ricin could not transfer across the healthy Caco-2 cell monolayer within three hours after poisoning. However, it could pass through the everted rat intestinal wall after 0.5 h of incubation. The toxin in the liver, spleen, lungs and kidneys of mice could be detected as early as 1 h after intoxication. The pathological results were in accordance with the cytotoxicities of ricin in Caco-2, HepG 2, H1299 and MDCK cells, indicating that though no significant symptom in mice could be observed within 3 h after ricin intoxication, important tissues, especially the kidneys, were being injured by the toxin and that the injuries were progressing. |
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| Keywords: | ricin immunohistochemistry alimentary poisoning |
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