三种生防细菌对新疆灰霉病菌(Botrytis cinerea)的拮抗作用

【目的】明确生防芽孢杆菌A57、A178、N1729在植物病害生物防治中的实用价值,并为后续的生防制剂的研发提供科学依据,对其在新疆各地蔬菜灰霉病菌的抑菌活性进行了研究。【方法】采用平板对峙法检测三种生防细菌抑菌活性,并检测其无细胞滤液的抑菌效果,无细胞滤液利用摇瓶发酵后经微孔滤膜过滤制备,用滤纸片法测定抑菌圈,确定抑菌效果。【结果】A178的活菌体拮抗效果最强,平均抑菌率达45.67%,N1729菌株分泌物的拮抗效果最强,平均抑菌率为44.61%,可作为灰霉病的有效生防菌;而A57的抑菌效果最差,不能作为后灰霉病菌有效的生防菌使用。【结论】A178、N1729这两个菌株具有很好的开发应用前景。

Antagonism of the Three Biocontrol Bacteria Strains on Botrytis cinerea in Xinjiang

Objective]A study on the biocontrol efficacy of the Bacillus strains A57,A178 and N1729 against 13 Botrytis cinerea isolates from the different area in Xinjiang, was performed for evaluating the future application significance of the three strains for the biological control of plant diseases,and providing scientific basis for the future research on their development.Method]The antagonism of three biocontrol bacteria were tested on PDA plate against 13 Botrytis cinerea isolates respectively. By the liquid fermenting and the tiny hole filters, the antagonism of free cell filtrates were detected on PDA plate. Result]The results demonstrated,the antagonistic ability of A178 strain was the strongest among three biocontrol bacteria,the average inhibition rate of the strain was 45.67%. The average inhibition rate of N1729 strain' free cell filtrates was 44.61%. A57 strain had the better antagonistic ability to the pathogen, but its free cell filtrates had no effect on the pathogen.Conclusion]These evidences showed that the two strains A178,N1729 were excellent biocontrol material against Botrytis cinerea with good prospects of development and application.