| 猪繁殖与呼吸综合征病毒江苏株的分离与鉴定 |
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| 引用本文： | 刘金彪,孟庆华,吴艳涛,高崧,彭大新,刘秀梵. 猪繁殖与呼吸综合征病毒江苏株的分离与鉴定[J]. 动物医学进展, 2009, 30(4) |
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| 作者姓名： | 刘金彪 孟庆华 吴艳涛 高崧 彭大新 刘秀梵 |
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| 作者单位： | 扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州,225009;盐城生物工程高等学校,江苏盐城,224051 |
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| 基金项目： | 国家支撑计划，江苏省农业三项工程项目 |
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| 摘 要： | 从暴发猪高热病的江苏盐城地区某养殖户送检病料中分离到一株致Marc-145细胞病变的病毒,细胞培养物裂解上清经浓缩、负染后电镜观察可见直径约50 nm,有囊膜的球形病毒粒子;间接免疫荧光试验结果表明,该分离株与美洲型猪繁殖与呼吸综合征病毒阳性血清反应发出强特异性荧光;以特异性引物对分离的病毒进行RT-PCR扩增于600 bp～700 bp出现单一条带,序列分析显示Nsp2缺失了87 bp.结果表明,该分离株为美洲型猪繁殖与呼吸综合征病毒变异株,暂命名为JYC.
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 分离 鉴定 RT-PCR |
Isolation and Identification of Porcine reproductive and respiratory syndrome virus Strain from Jiangsu Province |
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| LIU Jin-biao,MENG Qing-hua,WU Yan-tao,GAO Song,PENG Da-xin,LIU Xiu-fan. Isolation and Identification of Porcine reproductive and respiratory syndrome virus Strain from Jiangsu Province[J]. Progress In Veterinary Medicine, 2009, 30(4) |
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| Authors: | LIU Jin-biao MENG Qing-hua WU Yan-tao GAO Song PENG Da-xin LIU Xiu-fan |
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| Affiliation: | 1.Key Laboratory of Animal Infectious Disease;Ministry of Agriculture;College of Veterinary Medicine;Yangzhou University;Yangzhou;Jiangsu;225009;China;2.Yancheng Biological Engineering High School;Yancheng;224051;China |
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| Abstract: | A virus was isolated from a suspicious case in Yancheng,Jiangsu province,in which there were some outbreaks of high swine fever.The virus casused cytopathic effect in Marc-145 cell post infection with the suspicious samples.The morphology of the virus was spherical in shape with envelope diameter of approximately 50 nm,which was examined by electron microscope via negative staining in supernatant fluid of the infected cell culture lysis products.Results of indirect immunofluorescence assay showed that the v... |
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| Keywords: | RT-PCR |
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