红龙草叶片的组织培养及其植株再生

 建立了红龙草叶片再生体系。叶片外植体在培养基MS + 6-BA 1.0 mg/L + 4-PU 1.0 mg/L +NAA 0.1 mg/L上形成浅绿色愈伤组织, 20 d后愈伤组织诱导率达100%。约45.31%的愈伤组织在添加6-BA 1.0 mg/L和NAA 0.4 mg/L的MS培养基上分化出紫红色的不定芽, 约6%的愈伤组织在该培养基上产生出细小叶片和绿色变异幼芽。所产生的紫红色不定芽在1/2MS +NAA 0.4 mg/L的培养基上可全部生根,长成完整植株。再生植株的移栽成活率达85%以上。

Tissue Culture of Altemanthera ficoidea ''''Ruliginosa''''Leaf Explants and Its Plantlet Regeneration

An efficient regeneration protocol was developed through shoot regeneration from leaf explants of Altemanthera ficoidea'Ruliginosa'.The results showed that light green calli could be formed from all the leaf explants of cultured in vitro for 20 days on MS medium containing 6-BA 1.0 mg/L,4-PU(N-phenyl-N'-(2-chloro-4-pyridyl)urea)1.0 mg/L and NAA 0.1 mg/L.Adventitious shoots in purple could be developed from the calli at the frequency of 45.31% on MS medium supplemented with 1.0 mg/L 6-BA and 0.4 mg/L NAA.About 6% of the regenerated variants buds with green leaf-colour and tiny leaves were also observed to form on the same medium.Adventitious purple shoots could be rooted and formed regenerated plants on 1/2 MS medium supplemented with 0.4 mg/L NAA.The survival rates of regenerated plants were more than 85%.