| 老芒麦种质的醇溶蛋白遗传多样性研究 |
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| 引用本文： | 马啸,陈仕勇,张新全,周永红,白史且,刘伟. 老芒麦种质的醇溶蛋白遗传多样性研究[J]. 草业学报, 2009, 18(3): 59-66. DOI: 10.11686/cyxb20090309 |
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| 作者姓名： | 马啸 陈仕勇 张新全 周永红 白史且 刘伟 |
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| 作者单位： | 1.四川农业大学动物科技学院,四川 雅安 625014;2.四川农业大学小麦研究所,四川 成都 611130;3.四川省草原科学研究院,四川 成都 611731 |
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| 基金项目： | 四川省"十一五"牧草育种攻关项目和国家重点基础研究发展计划(973计划)项目 |
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| 摘 要： | 利用A-PAGE的醇溶蛋白标记,对来自亚洲和北美的86份老芒麦种质的遗传多样性和遗传关系进行了分析。电泳共检测到52条醇溶蛋白条带,其中47条为多态性条带,多态性带百分比达90.4%。种质间遗传相似系数的变幅为0.108~0.952,平均值为0.373。利用Shannon多样性指数反映了类似的结果,即供试种质间的多样性指数达到0.460的较高水平。基于多样性指数计算了老芒麦种质地理类群遗传分化程度,地理类群内和类群间的遗传变异分别占总变异的55.8%和44.2%,这表明种质间存在较高水平的遗传多样性。对供试种质和地理类群的聚类分析结果均显示,来源于青藏高原的种质与其他地理来源的种质具有较大的差异,可以分成明显的2支。这种聚类结果可能与老芒麦种质的地理来源和生态适应性有关。本研究结果可为老芒麦种质的收集保护及核心种质构建提供有益信息。
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| 关 键 词： | 老芒麦 醇溶蛋白 遗传多样性 地理类群 遗传分化 |
| 收稿时间： | 1900-01-01; |
Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus |
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| MA Xiao,CHEN Shi-yong,ZHANG Xin-quan,ZHOU Yong-hong,BAI Shi-qie,LIU Wei. Genetic diversity of Gliadin in worldwide germplasm collections of Elymus sibiricus[J]. Acta Prataculturae Sinica, 2009, 18(3): 59-66. DOI: 10.11686/cyxb20090309 |
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| Authors: | MA Xiao CHEN Shi-yong ZHANG Xin-quan ZHOU Yong-hong BAI Shi-qie LIU Wei |
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| Affiliation: | 1.The Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China; 2.Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 3.Sichuan Academy of Grassland Sciences, Chengdu 611731, China |
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| Abstract: | Gliadin markers based on A-PAGE were used to analyze genetic diversity and genetic relationships among eighty-six Elymus sibiricus accessions from Asia and North America.A total of 52 gliadin bands were scored,of which 47 were polymorphic(90.4%).The generated similarity matrix showed that the genetic diversity within the accessions was fairly high(average similarity index=0.373).Similarity values among the accessions ranged from 0.108 and 0.952.Similar results were obtained when genetic diversity was estima... |
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| Keywords: | Elymus sibiricus gliadin genetic diversity geographic groups genetic differentiation |
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