| 膜联蛋白B2基因的克隆及其真核表达载体的构建 |
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| 引用本文： | 王义会,闫强,王平,王凯慧,陈明勇.膜联蛋白B2基因的克隆及其真核表达载体的构建[J].动物医学进展,2007,28(3):31-33. |
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| 作者姓名： | 王义会 闫强 王平 王凯慧 陈明勇 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京,100094
2. 第二军医大学医学遗传学教研室,上海,200433 |
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| 摘 要： | 根据GenBank已登录的猪囊尾蚴膜联蛋白B2基因序列,设计合成1对特异性引物,应用反转录-聚合酶链反应(RT-PCR)技术从猪囊尾蚴中扩增出膜联蛋白B2基因,将其克隆至pcDNA3.1表达载体上,经酶切鉴定和基因测序表明,目的基因AnnexinB2已正确地整合至表达质粒中,成功构建了膜联蛋白B2基因的真核表达载体.
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| 关 键 词： | 膜联蛋白B2基因 真核表达载体 克隆 |
| 文章编号： | 1007-5038（2007）03-0031-03 |
| 收稿时间： | 2006-11-07 |
| 修稿时间： | 2006年11月7日 |
Cloning and Construction of Eukaryotic Expression Vector of Annexin B2 Gene |
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| WANG Yi-hui,YAN Qiang,WANG Ping,WANG Kai-hui,CHEN Ming-yong.Cloning and Construction of Eukaryotic Expression Vector of Annexin B2 Gene[J].Progress In Veterinary Medicine,2007,28(3):31-33. |
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| Authors: | WANG Yi-hui YAN Qiang WANG Ping WANG Kai-hui CHEN Ming-yong |
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| Abstract: | According to the published sequence of annexin B2 gene of Cysticercus cellulosae , a pair of prim ers were designed and synthesized. The annexin B2 gene was amplified by RT-PCR method from Cysticercus cellulosae, and cloned into pcDNA3. 1 vector. The annexin B2 gene was sequenced and compared with the published sequence of annexin B2 gene of Cysticercus cellulosae in the GenBank. The annexin B2 gene was successfully conformed into the expression plasmid. The eukaryotic expression plasmid containing annexin B2 gene was successfully constructed and was helpful for the studies of function of annexin B2. |
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| Keywords: | annexin B2 gene eukaryotic expression vector clone |
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