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黄曲霉毒素B1荧光定量快速检测试纸卡和仪器技术性能优化研究
引用本文:巩性涛,王培,肖理文,宋永泉,徐秀,赵皖. 黄曲霉毒素B1荧光定量快速检测试纸卡和仪器技术性能优化研究[J]. 粮食储藏, 2021, 0(1): 37-43
作者姓名:巩性涛  王培  肖理文  宋永泉  徐秀  赵皖
作者单位:山东省粮油检测中心;南京微测生物科技有限公司
摘    要:基于时间分辨荧光微球制备的黄曲霉毒素B1荧光定量快速检测试纸卡,通过两线及三线试纸卡灵敏度与重复性的对比,筛选出效果更优的三线试纸卡,并对三线试纸卡不同性能要求进行检测验证。结果表明,黄曲霉毒素B1三线试纸卡检出限与定量限符合要求,准确度、重复性、稳定性等均达到更优的效果,更能满足检测需求。

关 键 词:黄曲霉毒素B1  三线  时间分辨荧光免疫层析法  检出限  定量限  准确性  重复性

RESEARCH ON TECHNICAL PERFORMANCE OPTIMIZATION ON INSTRUCTION OF THE QUANTITATIVE&RAPID TEST STRIP FOR AFLATOXIN B1
Gong Xingtao,Wang Pei,Xiao Liwen,Song Yongquan,Xu Xiu,Zhao Wan. RESEARCH ON TECHNICAL PERFORMANCE OPTIMIZATION ON INSTRUCTION OF THE QUANTITATIVE&RAPID TEST STRIP FOR AFLATOXIN B1[J]. www.ysfri.ac.cn, 2021, 0(1): 37-43
Authors:Gong Xingtao  Wang Pei  Xiao Liwen  Song Yongquan  Xu Xiu  Zhao Wan
Affiliation:(Shandong Grain and Oil Testing Center,250101;Nanjing Microtest Biotechnology Co.,Ltd,210000)
Abstract:The quantitative&rapid test strip for Aflatoxin B1is prepared based on the time-resolved fluorescence microsphere.Through the comparison of sensitivity and repeatability of two-line and three-line strip,the better three-line strip was selected,and the different performance requirements of the three-line strip were tested and verified.The results showed that the detection limit and quantitative limit of Aflatoxin B1three-line strip met the requirements,and the accuracy,repeatability and stability of the strip were better,which could meet the requirements of detection.
Keywords:Aflatoxin B1  three line  time resolved fluorescence immunochromatography  detection limit  limit of quantification  accuracy  repeatability
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