山黧豆根腐病病原菌亚洲镰孢菌的分离鉴定

为了明确山黧豆(Lathyrus sativus)根腐病病原菌的分类地位,本研究于2018年8月在四川省南充市采集山黧豆根腐病病样,经组织分离法进行分离纯化,采用形态学和翻译延伸因子(Translation elongation factor 1-agene,EF-1α)基因序列分析相结合的方法对致病菌进行鉴定.结果表...

Isolation and Identification of Fusarium asiaticum Causing Lathyrus sativus Root Rot

In order to identify the pathogen causing root rot on Lathyrus sativus,the infected plants were collected during August of 2018 in Nanchong city,Sichuan province. The pathogen was isolated from tissue， single spore was purified,and pathogenicity was tested in the laboratory. Pathogenic isolates were identified by morphological characteristics and EF-1α gene sequence analysis. The results showed that 39 isolates were obtained from infected plant,of which three isolates including Shan-27,Shan-28 and Shan-29 had strong pathogenicity to L.sativus,accounting for 7.69%. The above three strains were all close to Fusarium graminearum species complex in morphology. Their translation elongation factor (EF-1α) were amplified by PCR,sequenced,and compared with Blast in GenBank. The results showed that their EF-1α were clustered in the same group with F. asiaticum, and their homology were more than 99%. Therefore,according to the morphological characteristics of the isolates and the sequence analysis of EF-1α gene,the three pathogenic isolates causing L.sativus root rot were identified as F. asiaticum.