芥子油苷对豌豆蚜生长、存活及保护酶的影响

为了明确芥子油苷对2种色型豌豆蚜生长、存活及体内保护酶的影响.本研究采用豌豆蚜(Acyrthosiphon pisum)全纯人工饲料薄膜饲养技术,通过添加不同浓度(CK:0 μmol·g-1;A1:0.94 μmol·g-1;A2:1.87 μmol·g-1;A3:2.86 μmol·g-1)的芥子油苷,测定了豌豆蚜的...

Effects of Glucosinolates on the Growth,Survival and Protective Enzymes of Pea Aphid(Acyrthosiphonpisum)

To clarify the effects of glucosinolates on the growth, survival and protective enzymes of two colored pea aphid(Acyrthosiphon pisum), the survival rate, the growth rate of relative average daily body weight and protective enzyme activity of pea aphids were determined by adding different concentrations of glucosinolates (CK:0 μmol·g^-1, A1:0.94 μmol·g^-1, A2:1.87 μmol·g^-1, A3:2.86 μmol·g^-1) using a pea aphid full-pure artificial diet film feeding technology. The results showed that the glucosinolates had a certain inhibitory effect on the growth and survival with two colored pea aphids. The rate of winged aphid, which was 46.0% in red aphid and 43.3% in green was increased gradually and reached the maximum at 2.86 mol/g. Meanwhile, the activities of Superoxide Dismutase(SOD), Peroxisome(POD) and Hydrogen Peroxidase(CAT) in aphids were significantly increased, however, the total amount of soluble protein was decreased. Moreover, compared with normal feeding pea aphid, the response level of SOD and CAT in red type pea aphid feeding with glucosinolin was significantly higher than that of POD, and the response level of POD in green was significantly higher than that of SOD and CAT. The results showed that the protective enzyme system in pea aphid to glucosinolates with different color types had different response mechanisms.