草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析

植物硝酸盐转运蛋白(Nitrate transporter,NRT)可有效转运NO3-,提升氮素利用效率.为了解析草地早熟禾(Poa pratensis)适应不同浓度及不同形态氮素、干旱胁迫机理,本研究以草地早熟禾为试材,对NRT1/PTR FAMILY 8.3基因进行克隆、生物信息学分析,并分析了不同浓度及不同形态氮...

Cloning and Expression Analysis of NRT1/PTR FAM ILY 8.3 Gene in Kentucky Bluegrass

As the main part of urban landscaping,lawn plays an important role in improving the ecological environment. Poa pratensis L. is a cool-season turfgrass with high quality. Poa pratensis L. has a high demand for nitrogen nutrients. Nitrate transporter (NRT) in plants can effectively transport NO₃^- and improve nitrogen-use efficiency. In this study,the NRT1/PTR FAMILY 8.3 gene of Midnight II was cloned and analyzed its bioinformation and expression under different nitrogen concentrations,nitrogen forms and drought treatments. The main results showed that the NRT1/PTR FAMILY 8.3 gene was cloned from Poa pratensis L.,which belonged to the major facilitator superfamily (MFS) and was highly homologous to the NRT1/PTR FAMILY 8.3 gene from Brachypodium distachyon. qRT-PCR results showed that the NRT1/PTR FAMILY 8.3 gene was highly expressed in roots and leaves of Poa pratensis L. After induction with different nitrogen forms at the same concentration,the expression of NRT1/PTR FAMILY 8.3 gene in the NaNO₃ treatment group was the highest. The expression of NRT1/PTR FAMILY 8.3 gene under nitrogen starvation and high-concentration nitrate nitrogen (0,15 mM NaNO₃) was higher compared with that under suitable nitrogen (7.5 mM NaNO₃). Drought stress promoted the expression of NRT1/PTR FAMILY 8.3 gene to a certain extent. The cloning and expression analysis results of Poa pratensis L. NRT1/PTR FAMILY 8.3 provided a theoretical basis for further exploring its regulatory mechanism in different nitrogen environments,determining its affinity to nitrate nitrogen,and cultivating high-quality turfgrass varieties with high nitrogen-use efficiency.