| 土壤嗜铁细菌C19的筛选、分子鉴定及其对炭疽菌和镰刀菌的拮抗作用 |
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| 引用本文： | 林超,余贤美,王春妮,李书莹,贺春萍,郑服丛. 土壤嗜铁细菌C19的筛选、分子鉴定及其对炭疽菌和镰刀菌的拮抗作用[J]. 热带作物学报, 2009, 30(1): 94-98 |
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| 作者姓名： | 林超 余贤美 王春妮 李书莹 贺春萍 郑服丛 |
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| 作者单位： | 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,热带农业有害生物检测监控中心,海南儋州,571737;中国热带农业科学院环境与植物保护研究所,热带农业有害生物检测监控中心,海南儋州,571737;农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州,571737;海南省热带农业有害生物检测监控重点实验室,海南儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目，国家科技支撑计划，国家公益性行业(农业)科研专项基金 |
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| 摘 要： | 利用LB液体培养基进行生物富集,采用CAS(Chrome Azurol S)检测平板法.从海南岛橡胶树等作物根际土壤中分离获得43个产嗜铁索细菌菌株.通过室内平板对峙培养法研究其中嗜铁能力较强的菌株C19对12个常见炭疽菌和镰刀菌的拮抗作用,结果表明.该菌株对Fusarium solani等9个菌株有不同程度的拮抗作用,显示该菌株具有较好的生防潜能.扩增菌株C19的16S rDNA序列,序列测定结果显示,该片段长度为1 525 bp,经Blastn搜索进行序列比对.该细菌为洋葱伯克霍尔德氏菌(Burkholderia cepacia).
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| 关 键 词： | 嗜铁细菌 CAS检测平板法 16SrDNA 分子鉴定 拮抗作用 |
Isolation of Siderophore Producing Bacteria C19 from the Soil and Analysis of Its Antagonism |
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| Lin Chao,Yu Xianmei,Wang Chunni,Li Shuying,He Chunping and Zheng Fucong. Isolation of Siderophore Producing Bacteria C19 from the Soil and Analysis of Its Antagonism[J]. Chinese Journal of Tropical Crops, 2009, 30(1): 94-98 |
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| Authors: | Lin Chao Yu Xianmei Wang Chunni Li Shuying He Chunping Zheng Fucong |
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| Affiliation: | Lin Chao1,2,Yu Xianmei2,3,4,Wang Chunni1,Li Shuying1,He Chunping2,Zheng Fucong1,21 College of Environment , Plant Protection,Hainan University,Danzhou Hainan 571737,2 Environment ,Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Baleful Organism Detection ,Monitor Centre for Tropical Agriculture,3 Ministry of Agriculture Key laboratory forDetection , Monitoring of Invasive Pest of Tropical Agriculture , Forestry,4Haina... |
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| Abstract: | Chrome Azurol Sulphonate(CAS) agar plate assay was used to screen the siderophore producing bacteria from the rhizosphere soil of tropical crops,such as rubber,palm,etc.after biological enrichment with LB liquid medium,and 43 isolates were obtained.Strain C19,which produced transparent orange halo,was selected to study its antagonistic activity via duel-culture test.The strain C19 had strong antagonism against the 12 pathogens of Fusarium,Colletotrichum and Magnaporthe grisea,which indicated that the C19 ha... |
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| Keywords: | 16SrDNA |
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