Localization of immunoglobulins and complement by the peroxidase antiperoxidase method in autoimmune and non-autoimmune canine dermatopathies |
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| Authors: | F M Moore S D White J L Carpenter E Torchon |
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| Institution: | 1. Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany;2. Laboratory Diagnostic Center, RWTH University Hospital Aachen, D-52074 Aachen, Germany;1. Yonsei University College of Medicine, Seoul, Korea;2. Inserm U954 and Department of Gastroenterology, Nancy University Hospital, Université de Lorraine, France;3. Luton & Dunstable University Hospital NHS Foundation Trust, Luton, United Kingdom;4. Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea;1. Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;2. Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan;3. Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan;4. Department of Laboratory Medicine and Pathology, The University of British Columbia, Canada;5. School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan;6. School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan;7. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;8. Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan;9. International Ph.D. Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;10. Pulmonary Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan |
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| Abstract: | Formalin-fixed, paraffin embedded skin biopsy specimens from 44 dogs with various dermatopathies were examined for the presence of immunoglobulins (IgG, IgM, IgA) and complement (C3) by the peroxidase antiperoxidase method (PAP). Final diagnoses of the skin diseases were determined by clinical findings, fungal and bacterial cultures, skin scrapings, serum endocrinologic tests, and histologic features of cutaneous biopsies. The dermatopathies included examples of pyoderma (folliculitis/furunculosis), demodecosis, sarcoptic mange, dermatophytosis, endocrine dermatopathy, and autoimmune skin disease (AISD). In the latter category, 7 cases of pemphigus foliaceus, 1 pemphigus vulgaris, 4 discoid lupus erythematosus (DLE) and 4 examples of indeterminate AISD were examined. Immunoglobulins, C3, or both, were localized in tissue sections from AISD (15/16), pyoderma (7/11), demodecosis (4/8) sarcoptic mange (3/3), and dermatomycosis (2/3). Endocrine dermatopathy biopsies were consistently negative for Ig and C3 (0/3). The pattern of localization was most often intercellular (31/44 positive biopsies) with basement membrane immunoreactivity in 4 cases of DLE, and 1 case of indeterminate AISD. There was no consistent correlation between histologic features of hematoxylin and eosin-stained biopsies and the presence of Ig and C3. Clinical outcome was similar in both Ig and C3 positive and negative cases of non-AISD dermatitis. The high percentage (95%) of positive results in AISD biopsies indicates the usefulness and sensitivity of the PAP method for the localization of Ig and C3. Because of the high percentage of Ig localization in pyoderma (73%), biopsy specimens with extensive inflammatory skin disease are not suitable for diagnosis of AISD. The PAP method is useful in the diagnosis of AISD in biopsy specimens with minimal inflammation. Caution is warranted in the interpretation of immunoreactivity independent of clinical and histologic information. |
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