| 青椒子叶培养及植株再生 |
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| 引用本文： | 曹冬孙 贾士荣. 青椒子叶培养及植株再生[J]. 园艺学报, 1993, 20(2): 171-175 |
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| 作者姓名： | 曹冬孙 贾士荣 |
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| 作者单位： | 中国农业科学院生物技术研究中心，中国农业科学院生物技术研究中心 北京 100081，北京 100081 |
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| 摘 要： |
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| 关 键 词： | 辣椒 青椒 组织培养 子叶培养 |
In Vitro Plant Regeneration of Sweet Pepper |
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| Cao Dongsun and Jia Shirong. In Vitro Plant Regeneration of Sweet Pepper[J]. Acta Horticulturae Sinica, 1993, 20(2): 171-175 |
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| Authors: | Cao Dongsun and Jia Shirong |
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| Abstract: | Two cultivars of sweet pepper, 'Zhongjiao 2' , and 'Shuangfeng' , were screened out of eight cultivars for their high capacity of regeneration. The shoot-bud differentiation frequency reached as high as 100% and each explant produced up to 11. 7 buds. Various factors influencing in vitro shoot-bud differentiation and the shoot elongation in particular, which was a major problem in pepper tissue culture, were investigated. Our experimental results showed that the seedling age was very important, i.e. the cotyledon explants taken from 12-day-old seedlings displayed the highest shoot-bud regeneration capacity. As far as culture medium was concerned, the shoot-buds elongated well on MS+ZT2+GA_3 2mg/l. Addition of coconut milk or casein hydrolysate also promoted shoot elongation. |
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| Keywords: | Capsicum annuum Cotyledon explants regeneration |
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