| 小麦胚乳贮藏蛋白高分子量谷蛋白亚基抗血清的制备 |
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| 引用本文： | 宋希云 高文淑. 小麦胚乳贮藏蛋白高分子量谷蛋白亚基抗血清的制备[J]. 中国农业大学学报, 1998, 3(5): 1-6 |
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| 作者姓名： | 宋希云 高文淑 |
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| 作者单位： | 山东莱阳农学院农学系!265200 |
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| 基金项目： | 国家自然科学基金!39570491 |
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| 摘 要： | 本文利用纯化的小麦胚贮藏蛋白高分子量谷蛋白亚基为抗原制备了８个不同的兔抗血清，并对这些抗血清与不同品种小麦贮藏蛋白的免疫化学反应进行了研究。结果表明；ＨＭＷ－ＧＳ具有较好的免疫原性，能够产生较高效价的抗血清；ＨＭＷ－ＧＳ抗血清与小麦胚服全蛋白具有较强的反应，但与醇溶蛋白反应较弱；ＨＭＷ－ＧＳ抗血清可用于小麦品质性状的测定，但不能有效地鉴别不同的ＨＭＷ－ＧＳ。
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| 关 键 词： | 抗血清 高分子量 谷蛋白亚基 小麦 |
Preparation of Antisera to High-Molecular-Weight Glutenin Subunits (HMW-GS) of Wheat Endosperm Storage Protein |
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| Song Xiyun,Gao Wenshu,Lu Dong,Huang Tiecheng,Liu Guangtian. Preparation of Antisera to High-Molecular-Weight Glutenin Subunits (HMW-GS) of Wheat Endosperm Storage Protein[J]. Journal of China Agricultural University, 1998, 3(5): 1-6 |
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| Authors: | Song Xiyun Gao Wenshu Lu Dong Huang Tiecheng Liu Guangtian |
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| Affiliation: | Dept. of Agronomy &. Plant Genetics and Breeding |
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| Abstract: | Eight strains of antisera to HMW-GS in wheat endosperm storage protein were prepared and their reaction with glutenin of different cultivars were studied. The main results showed that the antisera with high titer could be obtained by immunizing rabbits with HMW-GS. Antisera to HMW-GS could react strongly with total glutenin from different wheat cultivars, but their reacting with gliadin was very weak. The glutenin could be distinguished from gliadin by using the antisera, but the different HMW-GS could not. The antisera also be used to detect the quality parameters of wheat. |
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| Keywords: | antisera HMW-GS wheat |
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