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绣线菊18份材料观赏性状与RAPD标记的关联分析
引用本文:刘慧民,朱玉涛,车代弟,姜隆,陈庆山,吴凤芝.绣线菊18份材料观赏性状与RAPD标记的关联分析[J].园艺学报,2010,37(7):1125-1131.
作者姓名:刘慧民  朱玉涛  车代弟  姜隆  陈庆山  吴凤芝
作者单位:(1东北农业大学园艺学院,哈尔滨 150030;2东北农业大学农学院,哈尔滨 150030;3哈尔滨应用职业技术学院,哈尔滨 150078)
基金项目:黑龙江省自然科学基金项目 
摘    要:利用208个RAPD标记,通过关联作图对18份绣线菊(Spiraea L.)材料的基因组进行扫描,分析其群体结构,进行28个观赏性状观测值与标记的关联分析。结果表明:18份绣线菊材料可分为6个亚群;全群体共有23个标记与16个观赏性状关联,其中5个标记同时与2个或多个性状关联;发掘出观赏性状优异关联标记7条(s5-2、s152-6、s1216-1等)及其载体品种,这些标记与观赏性状中的花期(s5-2等2个)、彩叶期(s1049-9等2个)、开花生物学特征(s22-1等7个)、花色(s103-9等3个)、花瓣形态(s22-7)等性状关联;载体品种包括‘金焰’绣线菊、‘金山’绣线菊、珍珠绣线菊、日本绣线菊及石蚕叶绣线菊等。

关 键 词:绣线菊  观赏性状  RAPD标记  关联分析  
收稿时间:2009-10-09

Association Analysis of Ornamental Traits with RAPD Markers in 18 Portion Materials of Spiraea L.
LIU Hui-min,ZHU Yu-tao,CHE Dai-di,JIANG Long,CHEN Qing-shan,WU Feng-zhi.Association Analysis of Ornamental Traits with RAPD Markers in 18 Portion Materials of Spiraea L.[J].Acta Horticulturae Sinica,2010,37(7):1125-1131.
Authors:LIU Hui-min  ZHU Yu-tao  CHE Dai-di  JIANG Long  CHEN Qing-shan  WU Feng-zhi
Institution:(1College of Horticulture,Northeast Agriculture University,Harbin 150030,China;2College of Agriculture,Northeast Agriculture University,Harbin 150030,China;3Harbin Application Vocational Technology College,Harbin 150078,China)
Abstract:Using the 208 RAPD markers and association mapping in 18 portion materials of Spiraea L.,by scanning their genome in this study. We analyzed their population structure and performed the association analysis between RAPD markers and 28 ornamental traits. The results showed that:Eighteen portion materials of Spiraea L. were composed of 6 subpopulations;There were 23 RAPD markers associated with 16 ornamental traits in the population,there were 5 RAPD markers associated with 2 or more traits;Seven super association markers(s5-2,s152-6,s1216-1) of the ornamental traits and their carried species were detected. These markers associated with flower period(s5-2 total 2),colored- leaf period(s1049-9 total 2),flowering biological habit(s22-1 total 7),flower color(s103-9 total 3)and petal shape(s22-7)of ornamental traits. The carried species were S. bumalda‘Gold Mound’,S. bumalda‘Gold Flame’,S.thunbergii Blume,S. japonica Linn.and S. chamedryfolia L.
Keywords:Spiraea L    ornamental trait  RAPD marker  association mapping
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