野大麦幼根的愈伤组织诱导及植株再生

以野大麦（Hordeumbrevisubulatum（Trin．）Link）的幼根为外植体，采用MS培养基附加不同浓度的2，4－D诱导愈伤组织，结果以2，4－D4．0mg／L为最佳。以较低浓度2，4－D培养基进行愈伤组织的继代。在MS培养基附加2，4－D1．0mg／L＋6－BA0．1mg／L，以极低的频率分化出芽，再在附加IAA0．5mg／L＋6－BA2．0mg／L＋KT1．0mg／L＋Sucrose20g／L上的MS培养基上生根，最终再生成完整植株。

Tissue Culture of Root and Plant Regenerated from Hordeum brevisubulatum (Trin.) Link

Using young root of Hordeum brevisubulatum (Trin. ) Link as explant, calli were induced on basal medium MS with different concentrations of 2,4-D. Results showed the best concentiation was 4. 0 mg/L. Calli were cultured on media with low concentration of 2,4-D. After the low shoot regenerated frequency appeared on MS with 2,4-D 1. 0 mg/L + 6-BA 0. 1 mg/L, those calli took roots on MS with IAA 0. 5 mg/L + 6-BA 2.0 mg/L + KT 1 .0 mg/L + sucrose 20 g/L, at last the whole plant regenerated.