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重组质粒pDsRed1-C1-PinX1的建立及其在乳腺癌MCF-7细胞中的表达
引用本文:梁桑华,石嵘,周晖,夏巍,周珏宇,郑文岭,马文丽.重组质粒pDsRed1-C1-PinX1的建立及其在乳腺癌MCF-7细胞中的表达[J].湛江医学院学报,2013,31(3):245-248,252.
作者姓名:梁桑华  石嵘  周晖  夏巍  周珏宇  郑文岭  马文丽
作者单位:梁桑华 (南方医科大学基因工程研究所,广东广州,510515); 石嵘 (南方医科大学基因工程研究所,广东广州,510515); 周晖 (南方医科大学基因工程研究所,广东广州,510515); 夏巍 (南方医科大学基因工程研究所,广东广州,510515); 周珏宇 (南方医科大学基因工程研究所,广东广州,510515); 郑文岭 (南方医科大学基因工程研究所,广东广州,510515); 马文丽 (南方医科大学基因工程研究所,广东广州,510515);
基金项目:国家自然科学基金(项目编号:81101536),广州市科技计划珠江科技新星专项(项目编号:2011J2200070)广东省教育厅高校优秀青年人才培育项目(项目编号:LYM11038)
摘    要:目的构建PinX1真核表达载体,观察PinX1基因对乳腺癌MCF-7细胞生长和增殖的影响。方法重组质粒转染MCF-7细胞并筛选稳定表达系;用Real-timePCR检测PinX1基因mRNA的表达;MTT法检测转染前后细胞生长变化;平板克隆形成试验检测PinX1对MCF-7集落形成能力的影响。结果构建了真核表达载体PDsRed1-C1-PinX1的稳定表达系;转染后乳腺癌MCF-7细胞生长明显减缓,集落形成能力降低。结论 PinX1基因可抑制乳腺癌MCF-7细胞的生长和增殖。

关 键 词:乳腺癌  PinX1  增殖

Construction of recombinant plasmid pDsRedl-C1-PinX1 and its expression in breast carcinoma MCF-7 cells
LIANG Sang-hua,SHI Rong,ZHOU Hui,HU Zong,XIA Wei,ZHENG Wen-ling,MA Wen-li.Construction of recombinant plasmid pDsRedl-C1-PinX1 and its expression in breast carcinoma MCF-7 cells[J].Journal of Guangdong Medical College,2013,31(3):245-248,252.
Authors:LIANG Sang-hua  SHI Rong  ZHOU Hui  HU Zong  XIA Wei  ZHENG Wen-ling  MA Wen-li
Institution:(Institute of Genetic Engineering, South Medical University, Guangzhou 510515, China)
Abstract:Objective To observe the effect of PinX1 gene on the growth and proliferation of MCF-7 breast carcinoma cell line by constructing the eukaryotic expression vector ofPinX1. Methods MCF-7 ceils were transfected by recombinant plasmid and stable expression cells were then screened. PinX1 mRNA expression, growth and colony forming ability of MCF-7 cells were detected by Real-time PCR, MTT and plate clone formation assays, respectively. Results The constructed eukaryotic vector pDsRedl-C1-PinX1 expressed PinX1 stably. The growth and colony-forming ability of MCF-7 cells decreased after the transfection ofPinX1 gene. Conclusion PinX1 can inhibit the growth and proliferation of MCF-7 cells.
Keywords:breast cancer  PinX1 gene  proliferation
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