| 抑制番茄早疫病菌活性化合物的 高通量筛选方法研究 |
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| 引用本文： | 张晓,王满意,于淑晶,王力钟,李宝聚,赵卫光. 抑制番茄早疫病菌活性化合物的 高通量筛选方法研究[J]. 农药学学报, 2010, 12(4): 468-471. DOI: 10.3969/j.issn.1008-7303.2010.04.16 |
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| 作者姓名： | 张晓 王满意 于淑晶 王力钟 李宝聚 赵卫光 |
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| 作者单位： | 1.南开大学 元素有机化学国家重点实验室,天津 300071 |
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| 基金项目： | 国家自然科学基金(20772069);国家重点基础研究发展计划(973计划)项目(2010CB126105);元素有机化学国家重点实验室开放基金(0913)资助项目. |
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| 摘 要： | 以OD 630 值作为评价指标,在高通量筛选设备上建立了番茄早疫病菌Alternaria solania的高通量定量筛选方法,并对嘧菌酯、百菌清、 NFDA1 醚唑、霜脲氰、甲霜灵、代森锰锌、福美双和咪鲜胺8种常用杀菌剂对番茄早疫病菌孢子萌发和菌丝生长的抑制效果进行了评价,结果与孢子萌发法和目测筛选法所得结论基本一致。
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| 关 键 词： | 高通量筛选 杀菌活性 番茄早疫病菌 |
| 收稿时间： | 2010-06-28 |
| 修稿时间： | 2010-10-03 |
High throughput screening as a tool for discovering agrochemicals effective on Alternaria solania |
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| ZHANG Xiao,WANG Man-yi,YU Shu-jing,WANG Li-zhong,LI Bao-ju and ZHAO Wei-guang. High throughput screening as a tool for discovering agrochemicals effective on Alternaria solania[J]. Chinese Journal of Pesticide Science, 2010, 12(4): 468-471. DOI: 10.3969/j.issn.1008-7303.2010.04.16 |
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| Authors: | ZHANG Xiao WANG Man-yi YU Shu-jing WANG Li-zhong LI Bao-ju ZHAO Wei-guang |
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| Affiliation: | 1.The State Key Laboratory of Elemental-organic Chemistry,Nankai University,Tianjin 300071,China2.National Pesticide Engineering Research Center,Nankai University,Tianjin 300071,China3.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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| Abstract: | With OD630 value as an evaluation index,high-throughput screening method for discovering agrochemicals effective on tomato early blight pathogen was established by a micro plate spectrophotometer.The effect of eight widely-used fungicides(including azoxystrobin,chlorthalonil,difenoconazole,cymoxanil,metalaxyl,mancozeb,thiram and prochloraz) against Alternaria solani spore germination and mycelial growth was tested.The conclusion from high-throughput screening method was almost consistent with that from conventional spore germination method or visual observation. |
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| Keywords: | high-throughput screening fungical activity Alternaria solania |
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