| 蓖麻蚕蛹卵巢细胞的离体培养及其病毒感染试验 |
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| 引用本文： | 浦秋文 胡敏. 蓖麻蚕蛹卵巢细胞的离体培养及其病毒感染试验[J]. 蚕业科学, 1989, 15(1): 56-57 |
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| 作者姓名： | 浦秋文 胡敏 |
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| 作者单位： | 武汉大学病毒系(浦秋文，胡敏)，武汉大学病毒系(卢文筠) |
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| 摘 要： | 本文采用不同的培养液在体外培养蓖麻蚕蛹卵巢细胞,比较观察细胞生长,发现这些细胞能连续传代。开始细胞生长相当慢,13代以后,生长加快,趋于稳定。并且发现这些细胞对蓖麻蚕核型多角体病毒十分敏感。
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| 关 键 词： | 蓖麻蚕 蚕蛹 卵巢细胞 离体培养 |
The culture of pupal ovarl cell of Attacus ricini in vitro and infection expe- riment to virus. Sci. Seric. |
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| Pu Qiuwen. The culture of pupal ovarl cell of Attacus ricini in vitro and infection expe- riment to virus. Sci. Seric.[J]. Acta Sericologica Sinica, 1989, 15(1): 56-57 |
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| Authors: | Pu Qiuwen |
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| Abstract: | The paper compared the growth of Attacus ricini ovarian cell in different media. The date indicates that tke medium BML-TC100 is suitabl for culturing Pupal ovaries of Attacus ricini and Trypfose broth can stimulate the gtowth of the insect cell.Now we have gained the 17 passage cells 7 months.The cell was found to be very susceptible to Attacus ricini nuclear polyhedrosis virus. |
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| Keywords: | Attacus ricini Silkworm pupa Ovarl cell Culture in vttro |
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