花叶夹竹桃离体培养技术研究

通过正交试验等方法对花叶夹竹桃茎段组织培养技术进行了研究．结果表明：在江苏句容，花叶夹竹桃最佳取材时间为4月下旬．在优良母株上选取外植体，经过0．1％HgCl2消毒15min，灭菌成活率为78．8％；添加3．5％的白砂糖和增加培养容器的透气性，能很好地预防玻璃苗的发生；改良MS附加ZT，BA，NAA等植物生长调节剂可以有效地促进试管芽苗的增殖和生长，其中ZT1．5mg／L＋BA2．0mg／L十IBA0．1mg／L组合最适宜增殖与生长培养，增殖达5．6倍，生长高度为3．2cm；适宜的生根培养基为1／2MS＋IBA0．5mg／L＋NAA0．3mg／L＋Charcoal3000mg／L＋白砂糖2％，生根率可达92％；经生根的试管苗移栽于V（蛭石）：V（珍珠岩）：V（泥炭）为6：3：1的混合基质中，控制温度在23～30℃，相对湿度90％，保湿12～18d，其间适当遮荫，30d后成活率达88％以上。

Stem Segment Culture in Vitro of Nerium indicum cv. Variegata

This experiment concerning Nerium indicum cv. Variegata. segment culture in vitro was committed on the explants sterilization,vitreous shoots control, shoot multiplication and root induction. The results showed that： The latter of April was optimal collection time in vitro. After 0.1% HgCl2 sterilization for 15 min, the survival rate for the forthcoming culture accounted up to 78.8% ;addition of 3.5% sugar and improvement on air-permeation of culture containers could effectively control vitreous shoots;the modified MS media supplemented with ZT, BA and NAA could ameliorate the culture multiplication and growth, among which the combination of 1.5 mg/L ZT ＋ 2.0 mg/L BA ＋ 0.1 mg/L IBA was optimal, with the multiplication time was up to 5.6 and growth height up to 3.2 cm ; the rooting medium 1/2 MS ＋ 0.5 mg/L IBA ＋ 0.3 mg/L NAA ＋ 3 000 mg/L Charcoal ＋ 2% sugar was optimal,rooting percentage was up to 92% ;the root-developed plantlets transplanted in the substrate mixed with vermiculite, pearlite and peat（ volumn ratio of 6 ： 3 ： 1 ） at 23 - 30 ℃, relative humidity was 90% for 12 - 1 g d, favorably shaded,the survival rate could get over 88% after 30 d.