Molecular detection of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' Infection in cats by direct PCR using whole blood without DNA extraction |
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Authors: | Watanabe Masashi Hisasue Masaharu Souma Takehisa Ohshiro Shuichi Yamada Takatsugu Tsuchiya Ryo |
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Institution: | Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, Sagamihara City, Kanagawa, Japan. |
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Abstract: | Detection of hemotropic Mycoplasma spp. infection was attempted in cats by PCR using whole blood without DNA extraction. A total 46 of 54 (85%) cats with suspected Mycoplasma spp. infection showed a positive reaction, corresponding completely with the results of standard PCR testing. The direct PCR assay was sensitive enough to detect more than 0.0061% parasitemia for ;C. M. haemominutum' and 0.0075% parasitemia for M. haemofelis. These data indicate that the direct PCR assay might be sufficient for use as a tool in clinical examinations. |
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