水杨酸对铜胁迫下小麦种子萌发及抗氧化代谢的影响

目的]研究水杨酸（SA）促进铜胁迫下小麦种子的萌发及其对萌发过程中氧化胁迫的缓解效应。方法]采用不同浓度的CuCl2模拟金属铜胁迫梯度处理小麦种子,以小麦种子发芽率达到对照的50%时,为铜胁迫对小麦种子萌发的半抑制浓度;用SA预处理小麦种子6 h后,置于半抑制浓度的铜胁迫条件下,研究小麦种子的发芽状况,检测种子中的铜含量,并探究萌发的种子中过氧化物酶（POD）活性和过氧化氢酶（CAT）活性2个抗氧化指标的变化。结果]SA能显著促进铜胁迫下小麦种子的萌发;经SA预处理的种子中铜含量明显低于对照;经SA预处理的小麦种子中POD和CAT的活性均明显高于对照。结论]SA能促进小麦种子的萌发,提高小麦种子的抗氧化能力,同时还可以通过降低种子中的铜含量来缓解其对种子的胁迫效应。

Effects of Salicylic Acid on Wheat Seeds Germination and Anti-oxidative Metabolism under Copper Stress

Objective] Effects of salicylic acid(SA) on wheat seeds germination under Cu2+ stress and its anti-oxidative roles are investigated.Method] Different density of CuCl2 mimic metal copper stress is used to for the treatment of wheat seeds.When wheat seeds germination ratio is up to 50% of the comparison,it is the semi-inhibition density of copper stress to the germination of wheat seeds.Studying the germination of wheat seeds after using SA to pretreat for six hours and been set under copper stress with semi-inhibition density,the contents of copper in germinating wheat seeds,and activities of catalase(CAT) and POD in seeds germination are determined.Results] SA can significantly promote wheat seeds to germinate under copper stress.The content of Cu in wheat seeds after pretreated with SA is significantly lower than that of the comparison;the activities of POD and CAT in wheat seeds after pretreated with SA was obviously higher than those in control.Conclusion] SA can promote wheat seeds to germinate,enhance the capability of anti-oxidation in germinating wheat seeds and alleviate copper stress by lowering the level of copper in seeds.