豆科植物多花木蓝抑菌活性成分研究

活性追踪下,采用柱层析分离多花木蓝籽石油醚提取物中的抑菌活性物质。采用生长速率法和牛津杯法,选取稻瘟病菌、辣椒枯萎病菌、油菜菌核病菌、梨黑星病菌为测试真菌,青枯菌、大肠杆菌、金黄葡萄球菌、绿脓杆菌、枯草芽胞杆菌为测试细菌,测试了一级柱层析各流分的抑菌活性,测试结果显示:A-4和A-5流分对所有测试菌均具有一定的抑菌活性,对梨黑星病菌的抑制作用最好,抑制率分别达到77.66%和74.67%;对青枯菌的抑菌圈最大,分别为1.37cm和1.66cm。分离出2个活性化合物,经13 C、1 H NMR波谱方法鉴定,为BalansenateⅠ、Ⅱ,为首次从该植物中分离得到。生测结果显示2个化合物具有较低的抑菌活性,其对金黄葡萄球菌活性最好,但最小抑菌浓度(MIC)和最小杀菌(MBC)浓度均只为250mg/L和500mg/L。

Antimicrobial constituents of Indigofera amblyantha

Monochamus spp. are important quarantine pests, which can be found in imported wood and wood packages. The cuticular hydrocarbons from the cuticle of Monochamus urussovi and M.sutor were analyzed by gas chromatography and mass spectrometry (GC-MS) without solvent. The composition of the cuticular hydrocarbons was then determined by the area normalizing method. By searching the NIST database and comparison with standard mass spectra, nine constituents were identified in the cuticular hydrocarbons of M. urussovi, and eight in those of M. sutor. The principal components of M. urussovi and M.sutor cuticular hydrocarbons were straight and branched-chain, saturated and unsaturated long-chain hydrocarbons ranging from C24 to C44. Similar hydrocarbon patterns were found in M. urussovi and M. sutor and six constituents were found in both pests but with differences in quantity. n-tetracosane, n-hexatriacontane and n-tetratetracontane were detected only in M. urussovi, whereas 2-methylhexacosane and 9-hexacosene were found only in M. sutor.