蛋白质SDS-PAGE用于螺旋藻分类及突变体鉴定的研究

螺旋藻经SDS提取液提取、冷丙酮沉降后，所得蛋白液的SDS-PAGE图谱条带清晰、信息量大.对10株钝项螺旋藻(Spirulinaplatensis)Sp-D，Sp-Y，Sp-F，Sp-J，Sp-B，Sp-S，Sp-K，Sp-Z及其交变体Sp-Z(S)和Sp-Z(L)，所作的蛋白质SDS-PAGE分析表明：①螺旋藻蛋白带主要分布于31kD和188kD之间，各藻株间的蛋白质电泳图谱的特征性差异可作为螺旋藻分类的主要依据之一；②Sp-J和Sp-K的蛋白质SDS-PAGE图谱相同，从而进一步确证它们属同一品系；③Sp-Z(S)和Sp-Z(L)作为Sp-Z的耐盐突变体和直线形突变体，分别比Sp-Z多1条43kD及少1条87kD的蛋白带；④所建立的蛋白质SDS-PAGE分析方法可用于螺旋藻分类及突变体鉴定.

tudies on taxonomy and mutants identification by protein SDS-PAGE in Spirulina.

The SDS-PAGE patterns of proteins, which extracted from Spirulina platensis by extractant containing SDS and purified by -20℃ actone,were clear and had many bands. The SDS-PAGE analysis of Sp-D,Sp-Y ,Sp-F ,Sp-J ,Sp-B, Sp-S ,Sp-K ,Sp-Z and it' s mutants: Sp-Z(S) and Sp-Z (L) showed that: (1) The protein bands of Spirulina mainly distributed from 31 kD to 188 kD,the patterns among strains were similar, but there were some remarkable differences, which could be valuable in the systematic taxonomic study of Spirulina; (2) The same protein patterns of Sp-J and Sp-K further indicated that they belonged to same strain; (3) Sp-Z(S) and Sp-Z(L) are the mutants from Sp-Z. A new 43 kD protein appeared on the pattern of Sp-Z(S) which could grow splendidly in sea-water media. And the 87 kD protein disappeared on the pattern of Sp-Z(L) with linear filaments; (4) The methods of protein SDS-PAGE could be useful in the taxonomic study and mutant identification of Spirulina.