牛膝提取物对大鳞鲃生长、部分非特异性免疫指标及抗病性的影响

为研究牛膝提取物对大鳞鲃Barbus capito生长性能、部分非特异性免疫指标和抗病性的影响,采用单因子试验方法,选取450尾体质量为(98.92±2.25)g的大鳞鲃,随机分成5组,分别投喂牛膝提取物水平为0(对照)、0.05%、0.10%、0.20%、0.40%的5种试验饲料,养殖周期为60 d。结果表明:在饲料中添加0.20%的牛膝提取物,能够显著提高大鳞鲃体质量、增重率和特定生长率;添加0.10%的牛膝提取物,可显著增加大鳞鲃的头肾体指数和脾体指数(P0.05);与对照组相比,0.20%、0.40%牛膝提取物添加组大鳞鲃血清溶菌酶(LZM)和酸性磷酸酶(ACP)活力均有显著提高(P0.05);用嗜水气单胞菌攻毒后,0.20%牛膝提取物添加组大鳞鲃存活率最高。研究表明,牛膝提取物可提高大鳞鲃生长性能、非特异性免疫力和抗病性,建议饲料中牛膝提取物的添加水平为0.20%。

Effects of Achyranthes bidentata extracts on growth performance,partial non-specific immunity and disease resistance of bulatmai barbel Barbus capito

A total of 450 individuals of bulatmai barbel Barbus capito with body weight of (98.92±2.25) g were randomly allocated into a circulating water system and fed diets containing Achyranthes bidentata extracts ( ABE) at a rate of 0% (a control group), 0.05%, 0.10%, 0.20%, and 0.40% at water temperature of (25±1)℃ for 60 d to investigate effects of ABE on growth performance, partial non-specific immunity and disease resistance of bulat-mai barbel. The results showed that significantly higher body weight, weight gain ratio ( WGR) and specific growth rate ( SGR) were found in the fish fed the diets containing 0. 2% of ABE, and that significantly higher head kidney-somatic index and spleen-somatic index were observed in the fish fed the diets containing 0. 1% of ABE (P<0.05). There were significantly higher activities of acid phosphatase (ACP) and lysozyme (LZM) in the fish fed the diets containing 0.20% and 0.40% of ABE than in fish fed the control diet(P<0.05). A challenge test of bulatmai barbel by pathogen Aeromonas hydrophila revealed that the maximal survival rate was observed in the in-fected fish fed the diet containing 0. 20% of ABE. The findings indicate that ABE leads to significantly promote growth performance, partial non-specific immunity and disease resistance of bulatmai barbell, with appropriate ABE content of 0.20%.