| 西南凤尾蕨(Pteris wallichiana Agardh.)发育过程及其孢子的无菌繁殖 |
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| 引用本文： | 张开梅,石雷,李东,张宪春.西南凤尾蕨(Pteris wallichiana Agardh.)发育过程及其孢子的无菌繁殖[J].园艺学报,2008,35(1):94-98. |
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| 作者姓名： | 张开梅 石雷 李东 张宪春 |
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| 作者单位： | 1. 中国科学院植物研究所,北京,100093;中国科学院研究生院,北京,100049
2. 中国科学院植物研究所,北京,100093 |
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| 基金项目： | 中国科学院知识创新工程项目
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科技部科技基础条件平台建设计划 |
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| 摘 要： | 研究了西南风尾蕨的发育过程和孢子的无菌培养方法.结果如下:孢子萌发为书带蕨型,配子体发育为水蕨型.1/2MS培养基中萌发率可达83.3%;MS培养基中配子体成苗率可达55.4%;试管苗移栽成活率可达80%以上.认为利用孢子的无菌繁殖方法进行西南凤尾蕨的规模化生产具有可行性.
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| 关 键 词： | 西南凤尾蕨 孢子 无菌培养 凤尾蕨 发育过程 孢子萌发 无菌 繁殖方法 Sterile Culture Process 规模化生产 利用 移栽成活率 试管苗 成苗率 配子体发育 萌发率 培养基 结果 培养方法 西南风 研究 |
| 文章编号： | 0513-353X（2008）01-0094-05 |
| 收稿时间： | 2007-07-06 |
| 修稿时间： | 2007-11-22 |
Development Process and Spore Sterile Culture of Pteris wallichiana Agardh. |
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| ZHANG Kai-mei,SHI Lei,LI Dong,ZHANG Xian-chun.Development Process and Spore Sterile Culture of Pteris wallichiana Agardh.[J].Acta Horticulturae Sinica,2008,35(1):94-98. |
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| Authors: | ZHANG Kai-mei SHI Lei LI Dong ZHANG Xian-chun |
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| Abstract: | The development process and spore sterile culture of Pteris wallichiana Agardh. were studied respectively. The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type. The maximum germination rate was obtained in 1/2MS medium, which reached 83.3% ; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached 55.4% ; the transplant survival rate of sporophyte in vitro reached above 80%. The research indicates that the utilization of the spore Sterile culture of P. wallichiana for its large-scale production is possible. |
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| Keywords: | Pteris wallichiana Agardh spore sterile culture |
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