| 镉对凡纳滨对虾离体血细胞的毒性影响 |
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| 引用本文： | 冼健安,王安利,苗玉涛,李彬,潘训彬.镉对凡纳滨对虾离体血细胞的毒性影响[J].水生态学杂志,2012,33(1):112-115. |
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| 作者姓名： | 冼健安 王安利 苗玉涛 李彬 潘训彬 |
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| 作者单位： | 华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,广东省高等学校生态与环境科学重点实验室,广州510631 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 对凡纳滨对虾血细胞进行离体镉离子应激，Cd2+浓度为10-3-10-9M，在应激6h后取样，利用流式细胞术（FCM）检测血细胞的活性和活性氧（ROS）含量。结果显示，当Cd2+浓度为10-5M时，血细胞的ROS含量显著升高；当Cd2+浓度为10-3和10-4M时，血细胞的死亡率和ROS含量均显著上升。这些结果表明Cd2+的细胞毒性作用与其浓度相关，Cd2+浓度达到10-5M时即可诱导血细胞ROS的产生，但当Cd2+浓度达到10-4M及以上时，被诱导产生的ROS对细胞造成氧化伤害从而导致细胞死亡。
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| 关 键 词： | 镉，凡纳滨对虾，血细胞，流式细胞术 |
| 收稿时间： | 9/23/2011 9:49:53 PM |
| 修稿时间： | 9/23/2011 9:49:53 PM |
Toxic Effects of Cadmium on Haemocytes of Litopenaeus vannamei in Vitro |
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| xian jian an,wang an li,miao yu tao,li bin and pan xun bin.Toxic Effects of Cadmium on Haemocytes of Litopenaeus vannamei in Vitro[J].Journal of Hydroecology,2012,33(1):112-115. |
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| Authors: | xian jian an wang an li miao yu tao li bin and pan xun bin |
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| Institution: | College of Life Science, South China Normal University,College of Life Science, South China Normal University,College of Life Science, South China Normal University,College of Life Science, South China Normal University,College of Life Science, South China Normal University |
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| Abstract: | After 6 h in vitro exposure to 10-9-10-3 M Cd2+, cell viability and ROS production of Litopenaeus vannamei haemocytes were determined by flow cytometry. The results showed that ROS production of haemocytes was significantly induced by 10-5-10-3 M cadmium. Exposure to 10-4 and 10-3 M cadmium caused significant increase in both cell mortality and ROS production. These results showed a relationship between Cd2+ exposure dose and its cytotoxicity on shrimp haemocytes. ROS of haemocytes would be induced for Cd2+ above 10-5 M, but cell death caused by ROS only occurred for Cd2+ above 10-4 M. |
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| Keywords: | Cadmium Litopenaeus vannamei Haemocyte Flow cytometry |
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