| 利用SSR标记对14个海岛棉品种的聚类分析 |
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| 引用本文： | 李金荣,王小国,朱永军,张西英,张薇. 利用SSR标记对14个海岛棉品种的聚类分析[J]. 新疆农业科学, 2009, 46(2): 237 |
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| 作者姓名： | 李金荣 王小国 朱永军 张西英 张薇 |
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| 作者单位： | 石河子大学/新疆兵团绿洲生态农业重点实验室,新疆石河子,832003;三门峡职业技术学院/生化工程系,河南三门峡,472000 |
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| 基金项目： | 新疆生产建设兵团科技局博士资金项目 |
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| 摘 要： | 为了给海岛棉育种实践提供可靠的信息,采用SSR标记技术对14个已知来源的海岛棉品种的遗传背景进行了遗传多样性分析.从筛选出的41对有多态性的SSR引物中共扩增出280个等位位点,其中214个位点有多态性,占76.4;,每对引物可扩增出1～15个位点,平均6.83个等位位点.聚类分析表明,在SM相似系数0.64水平上14个海岛棉品种可聚成4类.实验证明SSR标记技术可为海岛棉育种中亲本选配,提供可靠的分子水平上的依据.
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| 关 键 词： | 海岛棉 SSR 聚类分析 遗传多样性 |
| 收稿时间： | 2009-02-25 |
Cluster analysis on 14 Sea-Island Cotton by SSRs |
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| LI Jin-rong,WANG Xiao-guo,ZHU Yong-jun,Zhang Xi-ying,ZHANG Wei. Cluster analysis on 14 Sea-Island Cotton by SSRs[J]. Xinjiang Agricultural Sciences, 2009, 46(2): 237 |
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| Authors: | LI Jin-rong WANG Xiao-guo ZHU Yong-jun Zhang Xi-ying ZHANG Wei |
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| Abstract: | The genetic relationships among 14 Sea-Island Cotton varieties were analyzed by SSRs to provide credible genetic information in breeding Sea-Island Cotton 280 distinctive loci in total were amplified f rom 41 pairs of SSR primers,and 214 were polymorphic,which accounted for 76.4 %.1 to 15 polymorphic loci could be amplified from each primer,with an average of 6.83.The 14 Sea-Island Cotton varieties could be classified into 4 groups at the level of SM=0.64 by cluster analysis.SSR markers proved to be the work of Sea-Island cotton breeding practice of matching parents,to provide a reliable basis for the molecular level. |
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| Keywords: | SSR |
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