海南省土壤拮抗放线菌分离方法初探

针对海南土样拮抗放线菌的分离，实验初步解决了土样中的细菌和真菌在放线菌分离培养中的污染问题。研究测定了细菌和真菌抑制剂K2Cr2O7的最佳使用浓度、土样稀释浓度、土样加热和风干处理以及土样的贮存时间等对放线菌分离培养的影响。结果表明：K2Cr2O7在一定的浓度范围内使用可以明显抑制细菌的生长，对放线菌的生长无明显的影响，浓度在50μg／ml时效果最佳。当K2Cr2O7 的使用浓度在70μg／ml时放线菌生长受到抑制，使用浓度在30μg／ml时对细菌的抑制效果不明显；土样溶液涂抹平板培养时，土样溶液浓度在10-3~10-4时平板内长出的菌落在30~40个间，菌落的重叠率在20%左右，分离效率较高，是理想的浓度；在土样放置一个星期的自然风干后，同时使用细菌抑制剂K2Cr2O7可得到较理想的培养效果；对土样进行加热处理后，可明显降低细菌的数量，但同时也会降低放线菌数量，分离效率过低。

Study of Isolation of Antabonistic Actinomyces from Soil in Hainan Province

During the isolation of antabonistic actinomyces from soil in Hainan province, we solved the issues of a contamination by bacteria and fungi. In study, we tested the best concentration of effective convenient, cheap inhibitor K2Cr2O7 that can impure antabonistic actinomyces, and the soil were treated separately by heating high temperature 120℃ and drying in air. The best soil store time of isolating the antabonistic actinomyces was also did. The results showed that the right concentration K2Cr2O7 is 50μg／ml, The antabonistic actinomyces strains is 30~40 when the soil diluting concentration in 10-3~10-4,the efficiency is best. The soil staying in air after a week’s isolated more antabonistic actinomyces strains than that Heated by high temperature 120℃.