云南省烟草花叶病毒和黄瓜花叶病毒外壳蛋白基因克隆及序列分析

以从云南省主要烟区烟草上分离到的烟草花叶病毒（Tobacco mosaic virus, TMV）和黄瓜花叶病毒（Cucumber mosaic virus, CMV）的RNA为模板，采用RT-PCR技术，对TMV云南分离物（TMV-YN）的外壳蛋白（Coat protein，CP）基因及3’端非编码区和CMV云南分离物（CMV-YNa）的CP基因进行了cDNA克隆和序列测定。结果表明，TMV-YN的cDNA全长684个碱基（EMBL登录号为AJ239099），通过GenBank进行同源性比较发现：其5'端的480个碱基为CP基因，编码159个氨基酸；3'端的204个氨基酸为非编码区；此CP基因与TMV-U1株系和TMV韩国普通株系核苷酸同源性均为100%。CMV-YNa的CP基因全长657个碱基（EMBL登录号为AJ239098），编码218个氨基酸；此CP基因与CMV亚组 I 的CMV-RB株系、CMV-117F株系和CMV-Y株系的核苷酸同源性分别为96%、93%和92%。

cDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province

The cDNA of coat protein gene and its 3' non coding region of tobacco mosaic virus and coat protein gene of cucumber mosaic virus isolated from the tobacco in Yunnan province was synthesized by RT PCR. The amplified cDNA fragments were then cloned and sequenced. The fragment of TMV YN comprises 684bp (EMBL accession number: AJ239099), including 480bp of CP gene encoding 158 amino acids and a 204bp of 3' non coding region. Comparison either the nucleotide sequence of CP gene or that of 3' non coding region demonstrated that the TMV YN is identical to the TMV U1 strain and TMV common strain in Korea, with 100% homology. The CP gene of CMV YNa consists of 657nt encoding 218 amino acid (EMBL accession number: AJ239098). The nucleotide sequence of CMV YNa has 96%, 93% and 92% nucleotide identities with CMV RB, CMV 117F and CMV Y, respectively.