|
|||||
|
|
| 组织工程脂肪的构建 | |
| 引用本文： | 邹运动,杨合生,欧阳燕,徐军军,马兆峰,胡刚,李裕强,金岩. 组织工程脂肪的构建[J]. 西北农业学报, 2008, 17(2): 59-63 |
| 作者姓名： | 邹运动 杨合生 欧阳燕 徐军军 马兆峰 胡刚 李裕强 金岩 |
| 作者单位： | 1. 西北农林科技大学动物医学院,陕西杨凌,712100;第四军医大学组织工程研发中心,陕西西安,710032 2. 西北农林科技大学动物医学院,陕西杨凌,712100 3. 第四军医大学组织工程研发中心,陕西西安,710032 |
| 基金项目： | 国家高技术研究发展计划(863计划)组织器官工程重大专项 |
| 摘 要： | 为探索构建组织工程脂肪的新方法,分离出转染GFP基因的小鼠脂肪间充质干细胞(ADSCs),将细胞、胶原结合碱性成纤维细胞生长因子(bFGF)的复合物注射到裸鼠皮下,6周后取材。试验组ADSCs经过2周成脂诱导,对照组ADSCs未经成脂诱导。组织学结果显示试验组形成典型的"网状"脂肪组织结构,对照组无明显的脂肪结构形成。RT-PCR证实试验组PPARγ2和C/EBPα基因的表达,对照组PPARγ2和C/EBPα基因不表达。ADSCs与bFGF混合,以胶原作为支架构建组织工程脂肪是可行的,为临床脂肪组织缺陷的治疗提供新的思路。 |
| 关 键 词： | 组织工程脂肪 脂肪间充质干细胞 碱性成纤维细胞生长因子 |
| 文章编号： | 1004-1389(2008)02-0059-05 |
| 收稿时间： | 2007-09-28 |
| 修稿时间： | 2007-09-28 |
Reconstruction of Adipose Tissue Engineering |
|
| ZOU Yun-dong,YANG He-sheng,OU YANG Yan,XU Jun-jun,MA Zhao-feng,HU Gang,LI Yu-qiang and JIN Yan. Reconstruction of Adipose Tissue Engineering[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(2): 59-63 | |
| Authors: | ZOU Yun-dong YANG He-sheng OU YANG Yan XU Jun-jun MA Zhao-feng HU Gang LI Yu-qiang JIN Yan |
| Affiliation: | College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi''an Shaanxi 710032, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi''an Shaanxi 710032, China;Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi''an Shaanxi 710032, China;Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi''an Shaanxi 710032, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi''an Shaanxi 710032, China |
| Abstract: | |
| Keywords: | Adipose tissue engineering Mesenchymal stem cells derived from adipose tissue Basic fibroblast growth factor(bFGF) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载全文 | |