| 海南长春花黄化病植原体的16S rDNA序列分析研究 |
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| 引用本文： | 车海彦,罗大全,符瑞益,叶莎冰,吴云锋. 海南长春花黄化病植原体的16S rDNA序列分析研究[J]. 植物病理学报, 2009, 39(2): 212-216 |
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| 作者姓名： | 车海彦 罗大全 符瑞益 叶莎冰 吴云锋 |
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| 作者单位： | 1 西北农林科技大学植物保护学院 陕西省农业分子生物学重点实验室, 杨凌 712100;2 中国热带农业科学院环境与植物保护研究所, 儋州 571737 |
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| 摘 要： | Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle's leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.
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| 关 键 词： | rDNA序列分析 植原体 长春花 黄化病 多年生草本植物 16S aster 海南 |
| 收稿时间： | 2008-03-25 |
Sequence analysis of 16S ribosomal DNA of phytoplasma associated with periwinkle yellows disease in Hainan |
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| CHE Hai-yan,LUO Da-quan,FU Rui-yi,YE Sha-bing,Wu Yun-feng. Sequence analysis of 16S ribosomal DNA of phytoplasma associated with periwinkle yellows disease in Hainan[J]. Acta Phytopathologica Sinica, 2009, 39(2): 212-216 |
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| Authors: | CHE Hai-yan LUO Da-quan FU Rui-yi YE Sha-bing Wu Yun-feng |
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| Affiliation: | 1 College of plant protection and Shaanxi Key Laboratory for Agriculture Molecule Biotechnology, Northwest Agricultural & Forestry University, Yangling 712100, China;2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
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| Abstract: | Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle's leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle. |
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| Keywords: | periwinkle yellows disease Phytoplasma 16S rDNA sequence analysis |
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