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| 疫霉侵染的辣椒cDNA文库的构建及非特异性脂转移蛋白cDNA的筛选 | |
| 引用本文： | 林明,贺俐,徐波,刘志钦,王育娜,官德义,马洪丽,何水林. 疫霉侵染的辣椒cDNA文库的构建及非特异性脂转移蛋白cDNA的筛选[J]. 福建农林大学学报(自然科学版), 2009, 38(2) |
| 作者姓名： | 林明 贺俐 徐波 刘志钦 王育娜 官德义 马洪丽 何水林 |
| 作者单位： | 1. 福建农林大学生命科学学院,福建,福州,350002 2. 福建农林大学作物科学学院,福建,福州,350002;井冈山大学生命科学学院,江西,吉安,343009 3. 福建农林大学作物科学学院,福建,福州,350002 |
| 基金项目： | 国家自然科学基金，福建省自然科学基金 |
| 摘 要： | 建立了辣椒疫霉侵染的辣椒幼苗cDNA文库,该原始文库含有1.5×106个独立克隆,插入片段约为0.5-2 kb.采用基于PCR技术的96孔文库筛选方法,从该cDNA文库中筛选出了1个应答辣椒疫霉侵染的候选基因的全长cDNA.经生物信息学分析,推测这个候选基因为非特异性脂转移蛋白(non-specific lipid-transfer protein,nsLTP)基因. |
| 关 键 词： | 辣椒 辣椒疫霉 cDNA文库 非特异性脂转移蛋白 |
Construction for cDNA library of pepper infected by Phytophthora capsici.,and the screening of nsLTP cDNA from library |
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| LIN Ming,HE Li,XU Bo,LIU Zhi-qin,WANG Yu-na,GUAN De-yi,MA Hong-li,HE Shui-lin. Construction for cDNA library of pepper infected by Phytophthora capsici.,and the screening of nsLTP cDNA from library[J]. Journal of Fujian Agricultural and Forestry University, 2009, 38(2) | |
| Authors: | LIN Ming HE Li XU Bo LIU Zhi-qin WANG Yu-na GUAN De-yi MA Hong-li HE Shui-lin |
| Affiliation: | 1.College of Life Science;2.College of Crop Science;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;China;3.College of Life Science;Jinggangshan University;Jian;Jiangxi 343009;China |
| Abstract: | The total RNA was extracted from young pepper infected by Phytophthora capsici.,and the cDNA library including more than 1.5×106 individual clones was constructed with SMARTTM cDNA construction kit.A positive candidate resistant gene cDNA was isolated by 96-hole screening method based on PCR.The results of bioinformatics analysis showed that the candidate nsLTP resistant gene could code the non-specific lipid-transfer protein. |
| Keywords: | pepper Phytophthora capsici. cDNA library non-specific lipid-transfer protein(nsLTP) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |