| Cre／loxp重组系统在转基因植物中删除标记基因的应用 |
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| 引用本文： | 郑雪琴.Cre／loxp重组系统在转基因植物中删除标记基因的应用[J].安徽农学通报,2011,17(1):48-49,68. |
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| 作者姓名： | 郑雪琴 |
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| 作者单位： | 福建师范大学生命科学学院,福建福州,350108 |
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| 摘 要： | 在植物遗传转化中，标记基因是必须的，但标记基因的存在引起人们对转基因食品安全性的关注。Cre／loxp重组系统可用于删除转基因植物中的标记基因，可以通过杂交或二次转化，或瞬时表达、诱导表达、组织特异表达重组酶来获得无标记基因的转基因植株。
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| 关 键 词： | Cre／loxp 转基因植物 标记基因 基因删除 |
Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants |
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| Zheng Xueqin.Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants[J].Auhui Agricultural Science Bulletin,2011,17(1):48-49,68. |
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| Authors: | Zheng Xueqin |
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| Institution: | Zheng Xueqin(College of life Sciences,Fujian Normal University,Fuzhou 350108,China) |
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| Abstract: | Selectable marker genes are necessary for plant genetic transformation,but cause attentions about the security of genetically modified food.To remove selectable marker genes from transgenic plants by Cre/loxp recombination system,there are generally several strategies:crossing with Cre transgenic plant or constitutive expression of recombinase by re-transformation of Cre gene,or application of transient,inducible or tissue-specific expression of recombinase. |
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| Keywords: | Cre/loxp |
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