Identification of European and Asian pears using EST-SSRs from <Emphasis Type="Italic">Pyrus</Emphasis> |
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Authors: | Nahla Bassil Joseph D Postman |
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Institution: | (1) United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Clonal Germplasm Repository (NCGR), 33447 Peoria Road, Corvallis, OR 97333, USA |
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Abstract: | Ten EST-SSRs previously isolated from Pyrus were used to identify 81 P. communis, 13 P. pyrifolia and 20 P. ussuriensis or P. × bretschneideri accessions. Cross-transference of these EST-SSRs was high in these species. PYC-008 and PYC-004 were the least informative
SSRs in each of the pear species and were monomorphic in P. pyrifolia while PYC-013, PYC-002 and PYC-009b were the most informative in all species. EST-SSRs were very valuable for identification
of incorrectly identified accessions, failed grafts and sets of synonyms in each of the species. Unsuspected relationships
were uncovered, including a parental relationship between ‘Anjou’ and ‘Farmingdale’, a clonal relationship between ‘Berger’
and ‘Bartlett’, and a very close relationship between ‘Beurre Superfin’ and ‘Doyenne du Comice’. One SSR marker was different
in one of three sports of ‘Doyenne du Comice’ (‘Doyenne du Comice Crimson Gem’) and in one of two sports of ‘Anjou’ (‘Gebhard
Red’ red skin sport of ‘Anjou’). UPGMA cluster analysis separated the pear accessions into a large European cluster and an
Asian group mostly according to common ancestry, geographical origin or time of ripening. High cross-transference of EST-SSRs
in Pyrus species is very valuable for germplasm management in such a highly diverse collection as found at the NCGR Pyrus genebank in Corvallis, OR. |
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