| 烟草体细胞胚胎诱导发生初报 |
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| 引用本文： | 白永富,郭文仪,陈学军,张 恒,马文广,晋 艳. 烟草体细胞胚胎诱导发生初报[J]. 云南农业大学学报(自然科学版), 2004, 19(1): 28-31 |
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| 作者姓名： | 白永富 郭文仪 陈学军 张 恒 马文广 晋 艳 |
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| 作者单位： | 云南烟草科学研究院农业研究所，云南烟草科学研究院农业研究所，云南烟草科学研究院农业研究所，云南烟草科学研究院农业研究所，云南烟草科学研究院农业研究所，云南烟草科学研究院农业研究所 云南玉溪653100，云南玉溪653100，云南玉溪653100，云南玉溪653100，云南玉溪653100，云南玉溪653100 |
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| 基金项目： | 云南省烟草专卖局(公司)资助项目(01A02) |
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| 摘 要： | 以烤烟云烟85叶片诱导的愈伤组织为材料,通过不同的激素浓度配比进行试验,研究其体细胞胚胎的发生能力。结果表明:云烟85体胚诱导的最高频率为61.1%,其体细胞胚发生的最佳激素配比是NAA 2.0 mg/L,BA 0.2 mg/L,从诱导出的体胚中观察到体胚几个主要发育阶段。
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| 关 键 词： | 普通烟草 体细胞胚胎发生 诱导 |
| 文章编号： | 1004-390X(2004)01-0028-04 |
| 收稿时间： | 2003-05-27 |
The Preliminary Report on Tobacco Somatic Embryogenesis |
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| BAI Yong-fu,GUO Wen-yi,CHEN Xue-jun,ZHANG Heng,MA Wen-guang,JIN Yan. The Preliminary Report on Tobacco Somatic Embryogenesis[J]. Journal of Yunnan Agricultural University, 2004, 19(1): 28-31 |
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| Authors: | BAI Yong-fu GUO Wen-yi CHEN Xue-jun ZHANG Heng MA Wen-guang JIN Yan |
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| Affiliation: | (Agricutural Institute of Yunnan Academy of Tobacco Science, Yuxi 653100, China) |
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| Abstract: | Somatic embryogenesis and plant regeneration were studied from induced calli of flue-cured tobacco leaves. Factors that influencing on the somatic embryogenesis of flue-cured tobacco (cv. Yunyan 85) were also studied. The results showed that the maximum frequency of somatic embryogenesis was 61.1% for Yunyan 85; the only culture medium for somatic embryogenesis of Yunyan 85 was MS medium supplemented with NAA(2.0 mg/L),BA(0.5 mg/L) and 2.0% sucrose. Multiple morphology of somatic embryo was also observed. |
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| Keywords: | Nicotiana tabacum L. somatic embryogenesis induce |
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