| 珍稀共生食用真菌松茸DNA指纹研究 |
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| 引用本文： | 曾东方,罗信昌,陈升明.珍稀共生食用真菌松茸DNA指纹研究[J].园艺学报,2000,27(3):223-225. |
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| 作者姓名： | 曾东方 罗信昌 陈升明 |
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| 作者单位： | 1. 华中农业大学应用真菌研究室,武汉430070
2. 中兴大学生命科学院,台中40227 |
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| 基金项目： | 国家自然科学基金资助项目! (39770 0 2 5 ) |
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| 摘 要： | 利用ＲＡＰＤ分析技术他中国主产区野生松茸的ＤＮＡＥ指纹图谱。从４０个随机引物中筛选到１６个扩增效果好、重复性稳定的引物以介导不同来源的２０个松茸子实体ＤＮＡ的扩增,均得到清晰可辨的ＤＮＡ指纹。分隔数据分析表明供试松茸在７２％相似水平上聚成一大类,支持关于云南、四川、吉林的松茸是一个相同物种的结论。
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| 关 键 词： | 食用菌 松茸 RAPD分析 DNA指纹图谱 聚类分析 |
| 修稿时间： | 1999-10-08 |
Studies on DNA Polymorphisms of Symbiotic Edible Fungi Tricholoma matsutake |
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| Zeng Dongfang,Luo Xinchang,Johannes S.M.Tschen.Studies on DNA Polymorphisms of Symbiotic Edible Fungi Tricholoma matsutake[J].Acta Horticulturae Sinica,2000,27(3):223-225. |
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| Authors: | Zeng Dongfang Luo Xinchang Johannes SMTschen |
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| Abstract: | The
DNA fingerprinting patterns of wild basidiocarps of matsutake in China were analyzed based
on RAPD in this study.16 primers with good amplification and replication were screened
from 40 primers.The 16 sets of DNA fingerprinting patterns were sharply made for 20
basidiocarps tested.Matsutakes tested were clustered together at 0.72 similarity level by
UPGMA cluster analysis,and were demonstrated to be the same species Tricholoma matsutake,distrubuted in Yunnan,Sichuan and Jilin provinces of
China. |
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| Keywords: | Symbiotic edible fungus Tricholoma matsutake RAPD analysis Identification DNA fingerprin- ting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
