| 盐穗木根际土壤中耐盐菌B6的分离与鉴定 |
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| 引用本文： | 王多兵,任伟,张富春,樊永红. 盐穗木根际土壤中耐盐菌B6的分离与鉴定[J]. 安徽农业科学, 2013, 41(3): 957-960,969 |
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| 作者姓名： | 王多兵 任伟 张富春 樊永红 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆乌鲁木齐,830046;新疆大学生命科学与技术学院,新疆乌鲁木齐,830046;新疆大学生命科学与技术学院,新疆乌鲁木齐,830046;新疆大学生命科学与技术学院,新疆乌鲁木齐,830046 |
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| 基金项目： | 新疆大学大学生创新性实验计划重点项目(No.XJU-SRT-211018);新疆自治区特殊环境重点实验室开放课题(XJYS0203-2012-01);新疆大学自然地理学博士后科研流动站资助;新疆大学自然科学基金项目(BS080120) |
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| 摘 要： | [目的]从新疆五家渠市耐盐碱植物盐穗木根际土壤中分离出兼性耐盐菌B6,并对其分别进行形态特征、生理生化特性分析及16S rDNA鉴定。[方法]采用真细菌16S rDNA通用引物PCR扩增B6菌株的16S rDNA,使用NCBI-Blast软件在GenBank数据库中对其进行同源性检索,使用MEGA4软件构建系统发育树。[结果]B6菌能在0～20%NaCl的条件下生长,为有芽孢的杆状菌;B6的16S rD-NA长度为1 446 bp,与多种地衣芽孢杆菌的16S rDNA序列同源性高达99%。[结论]系统发育树表明B6菌株与地衣芽孢杆菌的亲缘关系最近,结合生理生化分析,可判断B6菌株为地衣芽孢杆菌。
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| 关 键 词： | 耐盐菌 16S rDNA 鉴定 系统发育 地衣芽胞杆菌 |
Isolation and Identification of A Halotolerant Bacteria Strain B6 Isolated from Rhizosphere Microbe of Halostachys Caspica |
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| Affiliation: | WANG Duo-bing et al(College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046) |
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| Abstract: | The common primers for the 16S rDNA of eubacteria were used for polymerase chain reaction（PCR）. Homology analysis was made by searching in Genbank with NCBI-Blast and the phylogenetic tree was constructed with MEGA4. [ Result] Strain B6 can grow on the media containing 0 -20% NaC1, and was bacilli with spore forming. The amplified 16S rDNA fragment length was 1 446 bp and the result indicated that the 16S rDNA homologies were 99% between strain B6 and many kinds of Bacillus Licheniformises. [ Conclusion] The tree showed the clo- sest relationship was between strain B6 and Bacillus licheniformis. By analyzing the physio-biochemical characteristics of strain B6, strain B6 could be identified as a species of Bacillus licheniformis. |
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| Keywords: | Halotolerant bacteria 16S rDNA Identification Phylogenesis Licheniformises |
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