| 矮牵牛愈伤组织耐盐性研究 |
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| 引用本文： | 杨晓玲,郭金耀. 矮牵牛愈伤组织耐盐性研究[J]. 安徽农业科学, 2013, 0(2): 507+511-507,511 |
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| 作者姓名： | 杨晓玲 郭金耀 |
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| 作者单位： | 淮海工学院海洋学院,江苏连云港,222005;淮海工学院海洋学院,江苏连云港,222005 |
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| 基金项目： | 中央财政支持地方高校发展专项资金资助(CXTD34) |
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| 摘 要： | [目的]了解矮牵牛愈伤组织的耐盐能力。[方法]将矮牵牛嫩叶接种到8种不同盐浓度的MS培养基中培养,观察愈伤组织生长分化情况。[结果]当培养基盐度为0.4%时,矮牵牛愈伤组织诱导率与对照无差异;当培养基盐度为0.8%以下时,提高盐度能促进矮牵牛愈伤组织的芽分化率;当培养基盐度大于0.8%时,矮牵牛愈伤组织的生长分化受到抑制;当培养基盐度达到2.8%时,矮牵牛外植体全部死亡。[结论]矮牵牛愈伤组织可适应0.8%以下的盐度。
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| 关 键 词： | 矮牵牛 愈伤组织 耐盐性 |
Study on Salt-tolerance of Petunia hybrida Callus |
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| Affiliation: | YANG Xiao-ling et al(School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005) |
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| Abstract: | [Objective] To study the salt-tolerance ability of Petunia hybrida callus.[Method] The callus growth differentiation was observed by inoculating P.hybrida leaves into eight different salt concentrations MS medium for culture.[Result] In 0.4% salinity medium,P.hybrida callus induction rate is the same with control group;below 0.8%,improving salinity could promote bud differentiation rate of P.hybrida callus;above 0.8%,the growth differentiation could be inhibited;in the medium salinity reached 2.8%,P.hybrida explant all died.[Conclusion] P.hybrida callus can adapt to the salinity below 0.8%. |
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| Keywords: | Petunia hybrida Vilm Callus Salt-tolerance |
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