| 反相高效液相色谱法测定新疆不同品种葡萄干中的有机酸 |
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| 引用本文： | 袁辉,远辉,王建玲.反相高效液相色谱法测定新疆不同品种葡萄干中的有机酸[J].安徽农业科学,2013(2):817-818. |
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| 作者姓名： | 袁辉 远辉 王建玲 |
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| 作者单位： | 新疆产品质量监督检验研究院,新疆乌鲁木齐,830011 |
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| 摘 要： | 目的]测定新疆产不同品种葡萄干中有机酸的含量。方法]采用Symmetry ShieldTMRP18(4.6 mm×250 mm,5μm)色谱柱,以磷酸盐缓冲溶液(pH 3.0)为流动相,流速1.0 ml/min,柱温30℃,紫外检测波长210 nm,建立了葡萄干中酒石酸、苹果酸等4种有机酸的高效液相色谱定量分析方法,测定了新疆8种不同品种葡萄干中的酒石酸、苹果酸、柠檬酸和丁二酸的含量。结果]该方法相对标准偏差(RSD)0.57%～1.78%,回收率97.6%～101.2%,各种酸的线性相关系数r>0.999 7。结论]所建立的方法快速、灵敏度高、重复性好,可用于葡萄干中有机酸的测定。
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| 关 键 词： | 反相高效液相色谱 葡萄干 有机酸 |
Determination of Organic Acids Content in Different Varieties of Raisin in Xinjiang by HPLC |
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| Institution: | YUAN Hui et al(National Quality Supervision Test Center of Agricultural Byproducts,Urumqi,Xinjiang 830011) |
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| Abstract: | Objective] To determine organic acid content in different varieties of raisin in Xinjiang.Method] By using Symmetry ShieldTM RP18（4.6 mm × 250 mm,5 μm） column,with phosphate buffer solution（pH 3.0） as the mobile phase at a flow rate of 1.0 ml/min,column temperature 30 ℃,UV detection wavelength 210 nm,a quantitative reversed-phase high performance liquid chromatography（HPLC） method was established for the determination of tartaric acid and malic acid in raisin.The contents of tartaric acid,malic acid,citric acid,succinic acid in 8 varieties of raisin in Xinjiang were determined.Result] RSD is 0.57%-1.78%,recovery rate 97.6%-101.2%,linear correlation coefficient of each acid is r0.999 7.Conclusion] The method could be applied in determination of organic acids in grape with characteristics of rapid,good sensitive and repeatability. |
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| Keywords: | HPLC Raisin Organic acids |
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