猪发育期NPY-Y1和GPR54受体基因的表达规律研究

目的]研究猪发育期NPY-Y1和GPR54受体基因的发育性变化。方法]分别选取初生、60、120、初情期、180日龄的苏姜猪母猪各3头,采集下丘脑、垂体、卵巢组织样品,提取组织RNA,采用荧光定量PCR分析NPY-Y1、GPR54受体基因的发育性变化。结果]下丘脑、垂体与卵巢内NPY-Y1 mRNA从初生到初情期表达量逐渐下降,在初情期达到最低,初情期后呈上升趋势。在3种组织内,初情期与其他时期NPY-Y1 mRNA表达量均存在显著差异(P<0.05);GPR54 mRNA从初生到初情期表达量逐渐上升,在初情期达到最高,初情期后呈下降趋势。在3种组织内,初情期也与其他时期GPR54 mRNA表达量也均存在显著差异(P<0.05)。结论]猪发育期NPY-Y1基因与GPR54基因表达存在负相关性。

Study on the Regularity of NPY-Y1,GPR54 Acceptor Gene Development Expression in Development Stage of Pig

Objective] To study development expression of NPY-Y1 and GPR54 acceptor gene in development stage of pig.Method] Three female Sujiang pigs were selected in different stages of birth,60,120,puberty and 180 days,total RNA extracted from tissue samples of hypothalamus,pituitary and ovaries,development expression of NPY-Y1 and GPR54 gene were determined by quantitative RT-PCR.Result] The results showed that the expression abundance of NPY-Y1 mRNA was gradually descending from birth to puberty,and after puberty the expression abundance was ascending tendency in all three tissues,and the expression abundance at the stage of puberty was significant difference with the other stage（P0.05）.But the expression abundance of GPR54 mRNA was gradually ascending from birth to puberty,and after puberty the expression abundance was descending tendency in all three tissues,the expression abundance at the stage of puberty was also significant difference with the other stage（P0.05）.Conclusion] The expression abundance of GPR54 mRNA was associativity with NPY-Y1 mRNA in development stage of pig.